qPCR-based detection of Colletotrichum truncatum in soybean seeds

qPCR-based detection of Colletotrichum truncatum in soybean seeds

Soybean seed infected with Colletotrichum truncatum is an important source of primary inoculum for anthracnose epidemics. Based on differences in the GAPDH gene sequences of Colletotrichum species, one pair of species-specific primers, CtruncF1/CtruncR1, was designed to accurately detect C. truncatu...

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Bibliographic Details
Published in:Tropical plant pathology Vol. 45; no. 5; pp. 550 - 555
Main Authors: Júnior, Manoel B. S., Resende, Mário L. V., Pozza, Edson A., Botelho, Deila M. S., Cardoso, Acleide M. S., Siqueira, Carolina S., Machado, José C., Resende, Alexandre R. M., Silveira, Gustavo C. D., Guimarães, Sarah S. C.
Format: Journal Article
Language:English
Published: Cham Springer International Publishing 01-10-2020
Springer Nature B.V
Subjects:
Anthracnose
Biomedical and Life Sciences
Colletotrichum truncatum
Deoxyribonucleic acid
DNA
Epidemics
Fungi
Gene sequencing
Glyceraldehyde-3-phosphate dehydrogenase
Inoculum
Life Sciences
Plant Pathology
Primers
Seeds
Short Communication
Soybeans
Species
Specific primers
Diagnostic
Molecular detection
Plant disease
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Summary:Soybean seed infected with Colletotrichum truncatum is an important source of primary inoculum for anthracnose epidemics. Based on differences in the GAPDH gene sequences of Colletotrichum species, one pair of species-specific primers, CtruncF1/CtruncR1, was designed to accurately detect C. truncatum in soybean seed samples. The primers amplified only a single PCR band of 211 bp from C. truncatum . SYBR Green qPCR using these primers enabled the detection of DNA of the target fungus in inoculated soybean seeds and in naturally infested seeds. The sensitivity of the method was 0.000253 ng/μL of C. truncatum DNA template, with an efficiency of 1.78 and a Ct of 30.09. These species-specific primers may be useful for certification of soybean seeds aimed at avoiding introduction of the pathogen into soybean-producing regions.
ISSN:1983-2052
1982-5676
1983-2052
DOI:10.1007/s40858-020-00380-7