Evolution of Campylobacter jejuni of poultry origin in Brazil

Evolution of Campylobacter jejuni of poultry origin in Brazil

Campylobacter jejuni is the most common pathogen associated with foodborne diseases. Persistent presence of this pathogen contaminating the environment in slaughterhouses and chicken products have been reported worldwide. Although many efforts have been employed for reducing C. jejuni contamination,...

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Bibliographic Details
Published in:Food microbiology Vol. 82; pp. 489 - 496
Main Authors: Melo, Roberta T., Grazziotin, Ana Laura, Júnior, Edson C.Valadares, Prado, Renata R., Mendonça, Eliane P., Monteiro, Guilherme P., Peres, Phelipe A.B.M., Rossi, Daise A.
Format: Journal Article
Language:English
Published: England Elsevier Ltd 01-09-2019
Subjects:
Abattoirs - standards
Abattoirs - statistics & numerical data
Animals
Anti-Bacterial Agents - pharmacology
Antibiotic resistance
Brazil - epidemiology
Campylobacter Infections - epidemiology
Campylobacter Infections - microbiology
Campylobacter Infections - veterinary
Campylobacter jejuni - drug effects
Campylobacter jejuni - genetics
Campylobacter jejuni - isolation & purification
Campylobacter jejuni - pathogenicity
Campylobacteriosis
Chicken
Chickens
Drug Resistance, Bacterial
Food Microbiology
Genetic diversity
Genetic Variation
Microbial Sensitivity Tests
Poultry - microbiology
Poultry Diseases - epidemiology
Poultry Diseases - microbiology
Prevalence
Slaughterhouse
Virulence
Virulence Factors - genetics
Brazil
Chicken
Genetic diversity
Virulence
Antibiotic resistance
Campylobacteriosis
Slaughterhouse
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Summary:Campylobacter jejuni is the most common pathogen associated with foodborne diseases. Persistent presence of this pathogen contaminating the environment in slaughterhouses and chicken products have been reported worldwide. Although many efforts have been employed for reducing C. jejuni contamination, few studies have been conducted to understand the dynamics of C. jejuni in slaughterhouses over time. In this study, we evaluated the virulence, antibiotic resistance and genetic diversity profiles of 99 C. jejuni isolated from chilled chicken carcasses collected in Brazilian slaughterhouses during two distinct periods (2011–2012 and 2015–2016). The virulence profile was evaluated for the presence of flaA, ciaB, cadF, pldA and cdtABC genes. Antibiotic resistance was evaluated for amoxicillin-clavulanic acid, gentamicin, erythromycin and tetracycline. Genetic diversity was assessed using RAPD-PCR. The prevalence of C. jejuni was significantly reduced in 2015–2016 as well the number of antibiotic (and multidrug) resistant isolates, except for tetracycline. However, isolates from 2015 to 2016 showed higher prevalence of multiple virulence genes and genetic diversity profile compared to isolates from 2011 to 2012. During the studied period, stricter regulations to control pathogens in poultry farms and slaughterhouses were implemented in Brazil, which may have contributed to the profile variation observed due to changes of selective pressures on bacterial populations. •A total of 1070 chicken carcasses were assessed for C. jejuni prevalence over time.•C. jejuni prevalence was reduced, however, the pathogenic potential was increased.•The number of MDR isolates was reduced whereas the genetic diversity increased.•Changes were found after the establishment of more rigorous regulations in Brazil.
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ISSN:0740-0020
1095-9998
DOI:10.1016/j.fm.2019.03.009