Modulation by acute stress of chloride permeation across microdissected vestibular neurons membranes: different results in two rabbit strains and CRF involvement

Free hand isolation of adult rabbit vestibular Deiters’ neurons and dissection of their single membranes allows the study of their ionic permeability characteristics in a microchambers device. In the case of hare-like rabbits, the dissection of such membranes presents evidence of a high basal permea...

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Bibliographic Details
Published in:	Brain research Vol. 890; no. 2; pp. 255 - 260
Main Authors:	Rapallino, Maria V., Cupello, Aroldo, Hydén, Holger, Izvarina, Natalia L.
Format:	Journal Article
Language:	English
Published:	London Elsevier B.V 02-02-2001 Amsterdam Elsevier New York, NY
Subjects:	Acute Disease Animals Behavior, Animal - physiology Cell Membrane - drug effects Cell Membrane - metabolism Cell Membrane Permeability - drug effects Cell Membrane Permeability - physiology Chloride Channels - drug effects Chloride Channels - metabolism Corticotropin-Releasing Hormone - drug effects Corticotropin-Releasing Hormone - metabolism CRF Deiters’ neuron Excitability Habituation, Psychophysiologic - physiology Male Neurons - drug effects Neurons - metabolism Rabbit Rabbits Stress Stress, Physiological - metabolism Stress, Physiological - physiopathology Vestibular Nucleus, Lateral - drug effects Vestibular Nucleus, Lateral - metabolism α-h CRF [9–41], α-Helical CRF [9–41] IPSP, Inhibitory postsynaptic potential CRF, Corticotropin releasing factor CRF Rabbit Excitability Deiters’ neuron Neural basis of behavior GABA, γ-Aminobutyric acid Stress DIDS, 4,4′-Diisothiocyanato stilbene-2,2′-disulphonic acid
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Summary:	Free hand isolation of adult rabbit vestibular Deiters’ neurons and dissection of their single membranes allows the study of their ionic permeability characteristics in a microchambers device. In the case of hare-like rabbits, the dissection of such membranes presents evidence of a high basal permeation of labelled chloride, possibly related to mechanical disturbance of the plasma membrane-related cytoskeleton and activation of chloride channels. This did not apply to the laboratory strain of white New Zealand rabbits. However, membranes from hare-like rabbits which were stressed by being rotated on a platform before the experiment, behaved like those from the New Zealand strain. Vice versa, habituation to handling day after day of New Zealand rabbits resulted in a chloride permeation equal to that of unstressed hare-like rabbits. We propose that the stressful conditions result in the release of neurochemical messages to the vestibular Deiters’ cells which influence their electrophysiological behavior. The corticotropin releasing factor (CRF), a stress-related peptide present in the climbing fibers, actually blocks the basal chloride permeation across the Deiters’ membranes and this effect is partially reversed by its receptor antagonist, α-helical CRF [9–41].
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0006-8993 1872-6240
DOI:	10.1016/S0006-8993(00)03175-9