Search Results - "Izant, Jonathan G"

Constitutive and Conditional Suppression of Exogenous and Endogenous Genes by Anti-Sense RNA by Izant, Jonathan G., Weintraub, Harold

“…Plasmid DNA directing transcription of the noncoding (anti-sense) DNA strand can specifically inhibit the expression of several test genes as well as normal,…”
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Calcium Lability of Cytoplasmic Microtubules and Its Modulation by Microtubule-Associated Proteins by Schliwa, Manfred, Euteneuer, Ursula, Bulinski, Jeannette Chloe, Izant, Jonathan G.

“…Detergent-extracted BSC-1 monkey cells have been used as a model system to study the Ca2+sensitivity of in vivo polymerized microtubules under in vitro…”
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A microtubule-associated protein in the mitotic spindle and the interphase nucleus by Izant, Jonathan G, Weatherbee, James A, McIntosh, J. Richard

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Inhibition of thymidine kinase gene expression by anti-sense RNA: a molecular approach to genetic analysis by Izant, J G, Weintraub, H

“…As an alternative approach to classical genetic analysis, we are investigating the potential of anti-sense (nonsense) DNA strand transcription to inhibit gene…”
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Expression and analysis of the green fluorescent protein gene in the fission yeast Schizosaccharomyces pombe by Atkins, D, Izant, J G

“…This report demonstrates that the Aequorea victoria green fluorescence protein (gfp) gene product will fluoresce in the fission yeast Schizosaccharomyces pombe…”
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The role of calcium ions during mitosis. Calcium participates in the anaphase trigger by Izant, J G

“…Calcium-containing solutions were microinjected into dividing PtK1 cells to assess the effect of calcium ion concentration on the morphology and physiology of…”
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Xenopus annexin II (calpactin I) heavy chain has a distinct amino terminus by Izant, J G, Bryson, L J

“…We have isolated cDNAs encoding annexin II (calpactin I) heavy chain homologues from a Xenopus oocyte cDNA library. Two of the clones are full length, while…”
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Microtubule-Associated Proteins: A Monoclonal Antibody to MAP2 Binds to Differentiated Neurons by Izant, Jonathan G., McIntosh, J. Richard

“…Hybridomas that secret IgG reacting specifically with the brain microtubule-associated protein MAP2 have been prepared with spleen cells from BALB/c mice…”
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Comparison of three 3' non-coding regions in Schizosaccharomyces pombe expression vectors: efficiencies of transcription termination and mRNA 3'-end formation by Patrikakis, M, Izant, J.G, Atkins, D. (Johnson Research Pty Limited, Sydney (Australia). R.W. Johnson Pharmaceutical Research Inst.)

“…Analysis of an established Schizosaccharomyces pombe episomal shuttle vector suggested that inefficient transcription termination was deleterious to plasmid…”
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Antisense gene expression in yeast by Atkins, D, Arndt, G M, Izant, J G

“…The use of antisense and ribozyme RNA to modulate gene expression is emerging as an effective genetic technique. A compilation of successful antisense gene…”
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A Microtubule-Associated Protein Antigen Unique to Mitotic Spindle Microtubules in PtK1 Cells by Izant, Jonathan G., Weatherbee, James A., McIntosh, J. Richard

“…Microtubule-associated proteins (MAPs) that copurify with tubulin through multiple cycles of in vitro assembly have been implicated as regulatory factors and…”
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Gene regulation by antisense RNA in the fission yeast Schizosaccharomyces pombe by Arndt, G M, Atkins, D, Patrikakis, M, Izant, J G

“…This report describes experiments designed to demonstrate the suitability of the fission yeast Schizosaccharomyces pombe as a host for antisense RNA…”
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Invariance and Heterogeneity in the Major Structural and Regulatory Proteins of Chick Muscle Cells Revealed by Two-Dimensional gel Electrophoresis by Izant, Jonathan G., Lazarides, Elias

“…A two-dimensional gel electrophoresis system is used to investigate some of the properties of desmin, the major subunit of the 100- angstrom filaments from…”
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Microtubule-Associated Proteins of HeLa Cells: Heat Stability of the 200,000 mol wt HeLa MAPs and Detection of the Presence of MAP-2 in HeLa Cell Extracts and Cycled Microtubules by Weatherbee, James A., Sherline, Peter, Mascardo, Renato N., Izant, Jonathan G., Luftig, Ronald B., Weihing, Robert R.

“…One of the major groups of microtubule-associated proteins (MAPs) found associated with the microtubules isolated from HeLa cells has a molecular weight of…”
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The ade6 gene of the fission yeast as a target for antisense and ribozyme RNA-mediated suppression by Atkins, D, Patrikakis, M, Izant, J G

“…A genetic system for the analysis of antisense and ribozyme mechanisms is a much needed experimental tool, and yeast represent a favorable organism on which to…”
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Antisense "pseudogenetics" by Izant, J G

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Chimeric antisense RNAs by Izant, J G, Hoff, A M, Sardelli, A D, Ford, M

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