Expression of α-Fetoprotein and Prostate-specific Antigen Genes in Several Tissues and Detection of mRNAs in Normal Circulating Blood by Reverse Transcriptase-Polymerase Chain Reaction

Background: α-Fetoprotein (AFP) and prostate-specific antigen (PSA) in serum are widely used as tumor markers in the evaluation of prognosis and management of patients with hepatocellular carcinoma and prostate cancer, respectively. To establish the molecular diagnosis of cancer, reverse transcripta...

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Bibliographic Details
Published in:	Japanese journal of clinical oncology Vol. 28; no. 12; pp. 723 - 728
Main Authors:	Ishikawa, Tomoyoshi, Kashiwagi, Hironobu, Iwakami, Yoko, Hirai, Misako, Kawamura, Tomonori, Aiyoshi, Yuji, Yashiro, Toru, Ami, Yoshihiro, Uchida, Kazuhiko, Miwa, Masanao
Format:	Journal Article
Language:	English
Published:	England Foundation for Promotion of Cancer Research 01-12-1998
Subjects:	Adenoma, Pleomorphic - diagnosis Adult alpha-Fetoproteins - genetics alpha-Fetoproteins - metabolism Bile Duct Neoplasms - diagnosis Biomarkers, Tumor - blood Carcinoma, Hepatocellular - diagnosis Carcinoma, Transitional Cell - diagnosis Fetus - chemistry Humans Liver Neoplasms - diagnosis Male molecular diagnosis Pancreatic Neoplasms - diagnosis prostate-specific antigen Prostate-Specific Antigen - blood Prostate-Specific Antigen - genetics Prostatic Neoplasms - diagnosis Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - blood Salivary Gland Neoplasms - diagnosis tissue-specific expression Urinary Bladder Neoplasms - diagnosis α-fetoprotein
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Summary:	Background: α-Fetoprotein (AFP) and prostate-specific antigen (PSA) in serum are widely used as tumor markers in the evaluation of prognosis and management of patients with hepatocellular carcinoma and prostate cancer, respectively. To establish the molecular diagnosis of cancer, reverse transcriptase polymerase chain reaction (RT-PCR) for AFP and PSA was used to identify circulating cancer cells in the blood of cancer patients. Here, we examined the tissue-specificity of AFP and PSA and tested whether AFP and PSA are suitable targets in the detection of certain cancer cells by RT-PCR using peripheral blood samples. Methods: Tissue specificity of AFP and PSA was analyzed by Northern blotting and RT-PCR. Probes for AFP and PSA were hybridized with poly A+ RNAs from 50 human tissues. RT-PCR for AFP and PSA mRNA was performed using several cancerous tissues and normal tissues and peripheral blood cells from seven healthy volunteers. Results: Broad expression of AFP was observed in several tissues and a large amount of AFP mRNA was found in fetal liver. PSA was expressed in prostate, salivary gland, pancreas and uterus. By RT-PCR, AFP and PSA mRNA were detected in several tumors, including salivary pleomorphic adenoma, hilar bile duct carcinoma, pancreatic carcinoma, transitional cell carcinoma of urinary bladder and thyroid papillary carcinoma. Furthermore, AFP and PSA mRNAs were frequently detected by RT-PCR, even in peripheral blood cells from healthy volunteers. Conclusions: Neither AFP nor PSA showed tissue-specific expression. AFP and PSA mRNA were detected in several diseased and non-diseased tissues and normal circulating blood by RT-PCR.
Bibliography:	istex:C1C4A574CFA20675D9F1E9B121B2B1873E57416A ark:/67375/HXZ-JM7G4MLX-T ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0368-2811 1465-3621
DOI:	10.1093/jjco/28.12.723