Activation of ascorbate metabolism by nitrogen starvation and its physiological impacts in Arabidopsis thaliana

Redox homeostasis is crucial for plant acclimation to nutrient-deficient conditions, but its molecular mechanisms remain largely unknown. In this study, the effects of nutrient deficiencies on antioxidant systems in Arabidopsis thaliana were investigated. We found that ascorbate content in the plant...

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Bibliographic Details
Published in:	Bioscience, biotechnology, and biochemistry Vol. 86; no. 4; pp. 476 - 489
Main Authors:	Iwagami, Takumi, Ogawa, Takahisa, Ishikawa, Takahiro, Maruta, Takanori
Format:	Journal Article
Language:	English
Published:	England 21-03-2022
Subjects:	Antioxidants - metabolism Arabidopsis - metabolism Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Ascorbate Peroxidases - metabolism Ascorbic Acid - metabolism Gene Expression Regulation, Plant Nitrogen - metabolism ascorbate nitrogen starvation Arabidopsis high light oxidative stress
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Summary:	Redox homeostasis is crucial for plant acclimation to nutrient-deficient conditions, but its molecular mechanisms remain largely unknown. In this study, the effects of nutrient deficiencies on antioxidant systems in Arabidopsis thaliana were investigated. We found that ascorbate content in the plants grown with nitrogen starvation was higher than those with complete nutrition. The higher ascorbate levels were associated with enhanced gene expression of ascorbate biosynthesis enzymes and cytosolic isozymes of the ascorbate-glutathione cycle, suggesting that nitrogen starvation facilitated both consumption and biosynthesis of ascorbate. Nevertheless, we did not identify any phenotypic differences between wild type and ascorbate-deficient mutants (vtc2) under nitrogen starvation. Under high-light stress, the vtc2 mutants suffered severer photoinhibition than wild type. Interestingly, when high-light stress and nitrogen starvation were combined, wild type and vtc2 plants exhibited photoinhibition to the same extent. Based on these findings, we discuss the regulation and role of ascorbate metabolism under nitrogen starvation.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1347-6947 1347-6947
DOI:	10.1093/bbb/zbac010