Mineralized DNA-collagen complex-based biomaterials for bone tissue engineering

Mineralized DNA-collagen complex-based biomaterials for bone tissue engineering

DNA is a highly polyanionic biomolecule that complexes with both collagen and hydroxyapatite. By combining these complexes, we synthesized nucleic-acid collagen complexes (NACC) mineralized with hydroxyapatite. The composite complexes were made using a short, monodisperse single-stranded DNA, type I...

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Bibliographic Details
Published in:International journal of biological macromolecules Vol. 161; pp. 1127 - 1139
Main Authors: James, Bryan D., Guerin, Paxton, Iverson, Zion, Allen, Josephine B.
Format: Journal Article
Language:English
Published: Netherlands Elsevier B.V 15-10-2020
Subjects:
Biocompatible Materials - chemistry
Bone Regeneration
Cell Survival
Cells, Cultured
Chemical Phenomena
Collagen - chemistry
DNA - chemistry
DNA-collagen complex
Durapatite
Humans
Macromolecular Substances - chemistry
Macromolecular Substances - ultrastructure
Minerals - chemistry
Osteoblasts - cytology
Self-assembly
Spectrum Analysis
Tissue Engineering
Tissue Scaffolds - chemistry
Tissue engineering
Self-assembly
DNA-collagen complex
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Summary:DNA is a highly polyanionic biomolecule that complexes with both collagen and hydroxyapatite. By combining these complexes, we synthesized nucleic-acid collagen complexes (NACC) mineralized with hydroxyapatite. The composite complexes were made using a short, monodisperse single-stranded DNA, type I collagen, and mineralizing medium. They rapidly self-assembled into both mineralized NACC microfibers and 3D NACC gels. At the nanoscale, these complexes are hierarchical, interwoven, curly nanofibrils resembling native extracellular matrix, which mineralized an interpenetrating nanocrystalline hydroxyapatite phase. Mineralization was able to be done either before or after NACC formation enabling temporal control of the process. In response to the NACC material, primary human osteoblasts took on an osteocyte-like morphology. Moreover, the cells agglomerated and remodeled the NACC gels into densified, tissue-like structures within 3 days. NACC fibers and gels have promise not only as osteoconductive coatings and scaffolds, but as coatings and scaffolds for any tissue using this new form of naturally-derived biomaterials. [Display omitted] •Synthesized nucleic-acid collagen complexes (NACC) mineralized with hydroxyapatite•Rapidly self-assembled into both mineralized NACC microfibers and 3D NACC gels•Complexes are hierarchical, interwoven, curly nanofibrils.•Primary human osteoblasts took on an osteocyte-like morphology.•Osteoblasts remodeled the NACC gels into densified, tissue-like structures.
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ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2020.06.126