Hepatoprotective effects of Tagetes lucida root extract in carbon tetrachloride-induced hepatotoxicity in Wistar albino rats through amelioration of oxidative stress

The roots of Tagetes lucida Cav. (Asteraceae) have antioxidant and antimicrobial properties. This study aimed to examine the hepatoprotective effects of T. lucida roots ethanol extract (TLRE) using carbon tetrachloride (CCl 4 )-induced hepatotoxicity in rats. The active ingredients of TLRE were iden...

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Bibliographic Details
Published in:	Pharmaceutical biology Vol. 59; no. 1; pp. 984 - 995
Main Authors:	El-Newary, Samah Ali, Ismail, Rasha Fouad, Shaffie, Nermeen Mohammed, Hendawy, Saber Fayez, Omer, Elsayed, Ahmed, Mahgoub Mohammed, ELsayed, Wael M.
Format:	Journal Article
Language:	English
Published:	England Taylor & Francis 01-01-2021 Taylor & Francis Ltd Taylor & Francis Group
Subjects:	Alanine Alanine transaminase Alanine Transaminase - blood Alkaline phosphatase Animals Anti-inflammatory effect antioxidant characteristics Antioxidants Aspartate aminotransferase Aspartate Aminotransferases - blood Carbon Carbon tetrachloride Carbon Tetrachloride - pharmacokinetics Chemical and Drug Induced Liver Injury - drug therapy Chemical and Drug Induced Liver Injury - pathology Chromatography Clinical trials CYP2E1 inhibitor Cytochrome Cytochrome P-450 CYP2E1 - metabolism Cytochrome P450 Drug dosages Enzymes Ethanol Flavonoids Force-feeding Hepatotoxicity High-performance liquid chromatography Lipid peroxidation Lipids Lipids - blood Liver - pathology Male Metabolites Models, Animal Oral administration Oxidative stress Oxidative Stress - drug effects Plant Extracts - pharmacology Rats Rats, Wistar Research centers Seeds Silymarin Silymarin - pharmacology Tagetes - chemistry Tagetes lucida Toxicity Tumor Necrosis Factor-alpha - metabolism Tumor necrosis factor-α Tumors CYP2E1 inhibitor Anti-inflammatory effect antioxidant characteristics
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Summary:	The roots of Tagetes lucida Cav. (Asteraceae) have antioxidant and antimicrobial properties. This study aimed to examine the hepatoprotective effects of T. lucida roots ethanol extract (TLRE) using carbon tetrachloride (CCl 4 )-induced hepatotoxicity in rats. The active ingredients of TLRE were identified by high-performance liquid chromatography, infra-red spectrum, and mass spectrometric procedures. Ninety rats were distributed into four main groups: positive, therapeutic, protective, and negative group. The therapeutic group was implemented using CCl 4 (a single dose of 2 mL/kg) before TLRE or silymarin administration. Meanwhile, the protective group was implemented by administering CCl 4 (a single dose of 2 mL/kg) after force-feeding TLRE or silymarin. Each therapeutic and protective group was divided into three subgroups: force-fed with saline, TLRE (500 mg/kg), and silymarin (25 mg/kg). The positive group was split into two subgroups that were force-fed TLRE and silymarin. Positive, therapeutic, and protective groups were compared to the negative group (untreated rats). CCl 4, TLRE, and silymarin were orally administrated using a gastric tube. In the therapeutic and protective groups, TLRE significantly reduced liver enzymes, i.e., aspartate aminotransferase (12.47 and 6.29%), alanine aminotransferase (30.48 and 11.39%), alkaline phosphatase (17.28 and 15.90%), and cytochrome P450-2E1 (39.04 and 48.24%), and tumour necrosis factor-α (53.72 and 53.72%) in comparison with CCl 4 -induced hepatotoxicity controls. TLRE has a potent hepatoprotective effect with a good safety margin. After a repeated study on another type of small experimental animal, their offspring, and an experiment with a large animal, this study may lead to clinical trials.
ISSN:	1388-0209 1744-5116
DOI:	10.1080/13880209.2021.1949024