Effects of Platelet-Rich Plasma on Cellular Populations of the Central Nervous System: The Influence of Donor Age

Platelet-rich plasma (PRP) is a biologic therapy that promotes healing responses across multiple medical fields, including the central nervous system (CNS). The efficacy of this therapy depends on several factors such as the donor's health status and age. This work aims to prove the effect of P...

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Bibliographic Details
Published in:	International journal of molecular sciences Vol. 22; no. 4; p. 1725
Main Authors:	Delgado, Diego, Bilbao, Ane Miren, Beitia, Maider, Garate, Ane, Sánchez, Pello, González-Burguera, Imanol, Isasti, Amaia, López De Jesús, Maider, Zuazo-Ibarra, Jone, Montilla, Alejandro, Domercq, María, Capetillo-Zarate, Estibaliz, García Del Caño, Gontzal, Sallés, Joan, Matute, Carlos, Sánchez, Mikel
Format:	Journal Article
Language:	English
Published:	Switzerland MDPI 09-02-2021 MDPI AG
Subjects:	aging central nervous system growth factors microglia neurons platelet-rich plasma aging growth factors microglia neurons platelet-rich plasma central nervous system
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Summary:	Platelet-rich plasma (PRP) is a biologic therapy that promotes healing responses across multiple medical fields, including the central nervous system (CNS). The efficacy of this therapy depends on several factors such as the donor's health status and age. This work aims to prove the effect of PRP on cellular models of the CNS, considering the differences between PRP from young and elderly donors. Two different PRP pools were prepared from donors 65‒85 and 20‒25 years old. The cellular and molecular composition of both PRPs were analyzed. Subsequently, the cellular response was evaluated in CNS in vitro models, studying proliferation, neurogenesis, synaptogenesis, and inflammation. While no differences in the cellular composition of PRPs were found, the molecular composition of the Young PRP showed lower levels of inflammatory molecules such as CCL-11, as well as the presence of other factors not found in Aged PRP (GDF-11). Although both PRPs had effects in terms of reducing neural progenitor cell apoptosis, stabilizing neuronal synapses, and decreasing inflammation in the microglia, the effect of the Young PRP was more pronounced. In conclusion, the molecular composition of the PRP, conditioned by the age of the donors, affects the magnitude of the biological response.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Undefined-1 ObjectType-Feature-3 content type line 23
ISSN:	1422-0067 1661-6596 1422-0067
DOI:	10.3390/ijms22041725