Search Results - "Irth, H."

Online Magnetic Bead Dynamic Protein-Affinity Selection Coupled to LC−MS for the Screening of Pharmacologically Active Compounds by Jonker, N, Kretschmer, A, Kool, J, Fernandez, A, Kloos, D, Krabbe, J. G, Lingeman, H, Irth, H

“…The online, selective isolation of protein−ligand complexes using cobalt(II)-coated paramagnetic affinity beads (PABs) and subsequent liquid…”
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Protein digestion optimization for characterization of drug–protein adducts using response surface modeling by Switzar, L., Giera, M., Lingeman, H., Irth, H., Niessen, W.M.A.

“…The formation of drug–protein adducts in vivo may have important clinical and toxicological implications. Consequently, there is a great interest in the…”
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Derivatization of carboxylic acids with 4-APEBA for detection by positive-ion LC-ESI–MS(/MS) applied for the analysis of prostanoids and NSAID in urine by Kretschmer, A., Giera, M., Wijtmans, M., de Vries, L., Lingeman, H., Irth, H., Niessen, W.M.A.

“…In order to develop a generic positive ionization ESI LC–MS method for a variety of interesting substance classes, a new derivatization strategy for carboxylic…”
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Spreeta-based biosensor assays for endocrine disruptors by Marchesini, G.R., Koopal, K., Meulenberg, E., Haasnoot, W., Irth, H.

“…The construction and performance of an automated low-cost Spreeta™-based prototype biosensor system for the detection of endocrine disrupting chemicals (EDCs)…”
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Effect of the mobile phase composition on the separation and detection of intact proteins by reversed-phase liquid chromatography–electrospray mass spectrometry by Garcı́a, M.C., Hogenboom, A.C., Zappey, H., Irth, H.

“…Various buffers (ammonium acetate, ammonium formate, and ammonium hydrogencarbonate), acids (formic acid, acetic acid, heptafluorobutyric acid, and…”
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Sample preparation for peptides and proteins in biological matrices prior to liquid chromatography and capillary zone electrophoresis by Visser, N F C, Lingeman, H, Irth, H

“…The determination of peptides and proteins in a biological matrix normally includes a sample-preparation step to obtain a sample that can be injected into a…”
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High-performance liquid chromatography with on-line coupled UV, mass spectrometric and biochemical detection for identification of acetylcholinesterase inhibitors from natural products by Ingkaninan, K., de Best, C.M., van der Heijden, R., Hofte, A.J.P., Karabatak, B., Irth, H., Tjaden, U.R., van der Greef, J., Verpoorte, R.

“…A high-performance liquid chromatography (HPLC) method with on-line coupled ultraviolet (UV), mass spectrometry (MS) and biochemical detection for…”
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On-line Formation, Separation, and Estrogen Receptor Affinity Screening of Cytochrome P450-Derived Metabolites of Selective Estrogen Receptor Modulators by VAN LIEMPD, S. M, KOOL, J, NIESSEN, W. M. A, VAN ELSWIJK, D. E, IRTH, H, VERMEULEN, N. P. E

“…We have developed a fully automated bioreactor coupled to an on-line receptor affinity detection system. This analytical system provides detailed information…”
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Systematic development of an enzymatic phosphorylation assay compatible with mass spectrometric detection by de Boer, A R, Letzel, T, Lingeman, H, Irth, H

“…The enzymatic peptide phosphorylation by cAMP-dependent protein kinase A (PKA) was optimized and monitored by means of electrospray ionization mass…”
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Screening of natural products extracts for the presence of phosphodiesterase inhibitors using liquid chromatography coupled online to parallel biochemical detection and chemical characterization by Schenk, T, Breel, G J, Koevoets, P, van den Berg, S, Hogenboom, A C, Irth, H, Tjaden, U R, van der Greef, J

“…The ability to rapidly identify active compounds in a complex mixture (e.g., natural products extract) is still one of the major problems in natural products…”
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Trace Analysis of Proteins Using Postseparation Solution-Phase Digestion and Electrospray Mass Spectrometric Detection of Marker Peptides by Bruyneel, B, Hoos, J. S, Smoluch, M. T, Lingeman, H, Niessen, W. M. A, Irth, H

“…Analytical methodologies for the absolute quantitation of proteins typically include a digest step often using trypsin as the proteolytic enzyme. In the…”
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Metal-Complex Formation in Continuous-Flow Ligand-Exchange Reactors Studied by Electrospray Mass Spectrometry by Krabbe, J.G., de Boer, A.R., van der Zwan, G., Lingeman, H., Niessen, W.M.A., Irth, H.

“…Electrospray ionization mass spectrometry was used to investigate complex formation of different metal complexes in a continuous-flow ligand-exchange reactor…”
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On-line SPE–CE for the determination of insulin derivatives in biological fluids by Visser, N.F.C, van Harmelen, M, Lingeman, H, Irth, H

“…An on-line SPE–CE system is described for the determination of insulin derivatives in urine, serum and plasma. By combining techniques based on different…”
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A generic assay for phosphate-consuming or -releasing enzymes coupled on-line to liquid chromatography for lead finding in natural products by Schenk, T, Appels, N.M.G.M, van Elswijk, D.A, Irth, H, Tjaden, U.R, van der Greef, J

“…A generic continuous-flow assay for phosphate-consuming or -releasing enzymes coupled on-line to liquid chromatography (LC) has been developed. Operating the…”
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Analytical tools for the detection and characterization of biologically active compounds from nature by van Elswijk, D.A., Irth, H.

“…Nature has been recognized as a rich source of medicinal compounds for hundreds to thousands of years. Today, a vast range of drugs, which represent the…”
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HPLC coupled on-line to ESI-MS and a DPPH-based assay for the rapid identification of anti-oxidants in Butea superba by Nuengchamnong, N, Jong, C.F. de, Bruyneel, B, Niessen, W.M.A, Irth, H, Ingkaninan, K

“…A reversed-phase HPLC coupled on-line to a radical scavenging detection system and MS/MS was developed in order to combine separation, activity determination…”
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Screening for metal ligands by liquid chromatography–ligand-exchange–electrospray mass spectrometry by Krabbe, J.G., Lingeman, H., Niessen, W.M.A., Irth, H.

“…Electrospray ionization mass spectrometry is applied for the selective detection of metal ligands after a post-column continuous-flow ligand-exchange reaction…”
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On-line SPE–RP-LC for the determination of insulin derivatives in biological matrices by Visser, N.F.C., Lingeman, H., Irth, H.

“…An automated and on-line solid-phase extraction (SPE)–liquid chromatography (LC) procedure is described for the determination of insulin in biological…”
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Selective detection and identification of phosphorylated proteins by simultaneous ligand-exchange fluorescence detection and mass spectrometry by Krabbe, J.G., Gao, F., Li, J., Ahlskog, J.E., Lingeman, H., Niessen, W.M.A., Irth, H.

“…A ligand-exchange method for the detection and identification of phosphorylated peptides in complex mixtures is presented that is based on the characterization…”
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High-performance liquid chromatography coupled to enzyme-amplified biochemical detection for the analysis of hemoglobin after pre-column biotinylation by van Bommel, M.R., de Jong, A.P.J.M., Tjaden, U.R., Irth, H., van der Greef, J.

“…The determination of proteins with enzyme-amplified biochemical detection (EA-BCD) coupled on-line with high-performance liquid chromatography (HPLC) is…”
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