Comparative characteristics of human interleukin-2 preparations obtained from various sources
Interleukin-2 (IL-2) was produced from donor peripheral blood lymphocytes and JURKAT FHCRC T lymphoma cells. As a result of the purification, the specific activity of the preparation increased by a factor of 400. It was shown that optimum proliferation of T lymphocytes requires the successive action...
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| Published in: | Biochemistry (Easton) Vol. 51; no. 8; pp. 1153 - 1158 |
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| Main Authors: | , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
01-01-1987
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| Online Access: | Get full text |
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| Summary: | Interleukin-2 (IL-2) was produced from donor peripheral blood lymphocytes and JURKAT FHCRC T lymphoma cells. As a result of the purification, the specific activity of the preparation increased by a factor of 400. It was shown that optimum proliferation of T lymphocytes requires the successive action of phytohemagglutinin and IL-2, as well as the presence of serum in the medium. The properties and methods of production of a long-proliferating line of IL-2-dependent T cells B-5 are described. The proliferation of B-5 cells depends completely on the presence of IL-2 in the medium, although prolonged proliferation requires periodic stimulation by antigen (allogeneic lymphocytes). In the absence of IL-2 in the medium, B-5 cells die within 36 h. The prospects for the use of IL-2 preparations from human peripheral blood lymphocyte culture fluid for adoptive immunotherapy of tumors and the use of cells of the IL-2-dependent line B-5 in the testing of the activity of IL-2 preparations obtained from various sources are discussed. |
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| Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 content type line 23 ObjectType-Feature-1 |
| ISSN: | 0006-2960 |