Performance and validation of an adaptable multiplex assay for detection of serologic response to SARS-CoV-2 infection or vaccination

Measurement of quantitative antibody responses are increasingly important in evaluating the immune response to infection and vaccination. In this study we describe the validation of a quantitative, multiplex serologic assay utilising an electrochemiluminescence platform, which measures IgG against t...

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Bibliographic Details
Published in:	Journal of immunological methods Vol. 510; p. 113345
Main Authors:	Kenny, Grace, Negi, Riya, O'Reilly, Sophie, Garcia-Leon, Alejandro, Alalwan, Dana, Gaillard, Colette Marie, Saini, Gurvin, Inzitari, Rosana, Feeney, Eoin R., Yousif, Obada, Cotter, Aoife G, de Barra, Eoghan, Sadlier, Corinna, Crispie, Fiona, Doran, Peter, Gautier, Virginie, Mallon, Patrick W.G.
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 01-11-2022 The Authors. Published by Elsevier B.V
Subjects:	Antibodies, Viral COVID-19 COVID-19 - diagnosis COVID-19 Testing Humans Immunoglobulin G SARS-CoV-2 Sensitivity and Specificity Serology Spike Glycoprotein, Coronavirus Vaccination COVID-19 SARS-CoV-2 Serology
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Summary:	Measurement of quantitative antibody responses are increasingly important in evaluating the immune response to infection and vaccination. In this study we describe the validation of a quantitative, multiplex serologic assay utilising an electrochemiluminescence platform, which measures IgG against the receptor binding domain (RBD), spike S1 and S2 subunits and nucleocapsid antigens of SARS-CoV-2. The assay displayed a sensitivity ranging from 73 to 91% and specificity from 90 to 96% in detecting previous infection with SARS-CoV-2 depending on antigenic target and time since infection, and this assay highly correlated with commercially available assays. The within-plate coefficient of variation ranged from 3.8–3.9% and the inter-plate coefficient of variation from 11 to 13% for each antigen.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0022-1759 1872-7905
DOI:	10.1016/j.jim.2022.113345