Effect of pgsE expression on the molecular weight of poly(γ-glutamic acid) in fermentative production

Effect of pgsE expression on the molecular weight of poly(γ-glutamic acid) in fermentative production

Poly(γ-glutamic acid) (PGA) is a biopolymer produced by Bacillus spp. via the γ-amide linkages of d - and/or l -glutamate. Although high-molecular-weight (HMW) PGA possesses many attractive properties, such as flocculating, wound healing, and immune-stimulating effects, no studies have reported fact...

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Bibliographic Details
Published in:Polymer journal Vol. 53; no. 2; pp. 409 - 414
Main Authors: Fujita, Ken-Ichi, Tomiyama, Takashi, Inoi, Takahiro, Nishiyama, Takashi, Sato, Eriko, Horibe, Hideo, Takahashi, Ryosuke, Kitamura, Shinichi, Yamaguchi, Yoshihiro, Ogita, Akira, Tanaka, Toshio
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 01-02-2021
Nature Publishing Group
Subjects:
631/326
631/61/54
Biomaterials
Bioorganic Chemistry
Biopolymers
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Chromatography
Flocculation
Glutamic acid
Light scattering
Linkages
Microorganisms
Molecular weight
Polymer Sciences
Productivity
Proteins
Size exclusion chromatography
Surfaces and Interfaces
Thin Films
Wound healing
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Summary:Poly(γ-glutamic acid) (PGA) is a biopolymer produced by Bacillus spp. via the γ-amide linkages of d - and/or l -glutamate. Although high-molecular-weight (HMW) PGA possesses many attractive properties, such as flocculating, wound healing, and immune-stimulating effects, no studies have reported factors useful for increasing the molecular weight of PGA during microbial production. PgsB, PgsC, and PgsA are the minimum protein sets required for PGA production in B. subtilis , and PgsE improves PGA productivity. Analysis by size-exclusion chromatography combined with multiangle laser light scattering revealed that the molecular weight of PGA was M w  = 2,900,000 g mol −1 and predominantly M w  = 47,000 g mol −1 in preparations derived from B. subtilis cells with and without pgsE , respectively. PgsE may be required to increase the molecular weight of PGA. Poly(γ-glutamic acid) (PGA) is a biopolymer produced by Bacillus spp. via the γ-amide linkages of d - and/or l -glutamate. PgsB, PgsC, and PgsA are the minimum protein set required for PGA production in B. subtilis , and PgsE improves PGA productivity. Analysis by size-exclusion chromatography combined with multiangle laser light scattering revealed that the molecular weight of PGA was M w = 2,900,000 g mol −1  or predominantly M w = 47,000 g mol −1  in preparations derived from B. subtilis cells with or without pgsE , respectively. PgsE may be required to increase the apparent molecular weight of PGA.
ISSN:0032-3896
1349-0540
DOI:10.1038/s41428-020-00413-7