Nematicidal activity of native Bacillus thuringiensis against the root knot nematode, Meloidogyne incognita (Kofoid and White)

Nematicidal activity of native Bacillus thuringiensis against the root knot nematode, Meloidogyne incognita (Kofoid and White)

In this study, potential of the native Bacillus thuringiensis Berliner was investigated against the root knot nematode, Meloidogyne incognita (Kofoid and White) under laboratory and greenhouse conditions. Fourteen out of 50 Bt isolates obtained from root zone of vegetable crops of north western zone...

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Bibliographic Details
Published in:Egyptian journal of biological pest control Vol. 30; no. 1; pp. 1 - 9
Main Authors: Ramalakshmi, A., Sharmila, R., Iniyakumar, M., Gomathi, V.
Format: Journal Article
Language:English
Published: Berlin/Heidelberg Springer Berlin Heidelberg 08-07-2020
Springer Nature B.V
SpringerOpen
Subjects:
Bacillus thuringiensis
Biological control
Biomedical and Life Sciences
Chemical treatment
CRY protein
Crystals
Hatching
Life Sciences
Meloidogyne incognita
nematicidal cry genes
Nematodes
Proteins
Root zone
Tomatoes
Zoology
genes
Biological control
nematicidal
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Summary:In this study, potential of the native Bacillus thuringiensis Berliner was investigated against the root knot nematode, Meloidogyne incognita (Kofoid and White) under laboratory and greenhouse conditions. Fourteen out of 50 Bt isolates obtained from root zone of vegetable crops of north western zone of Tamil Nadu, India, were found to be encoded with nematicidal cry genes, confirmed by gene-specific PCR. Nine different Cry protein profiles were obtained from native Bt . Spore-crystal mixtures of 6 isolates out of 14 (at 0.5%) showed 100% inhibition to J2 juveniles emergence from egg masses of M. incognita within 72 h of treatment. Analysis by SEM revealed that fluffy egg masses were observed in untreated samples due to hatching, whereas egg masses were covered by gelatinous matrix, when treated with spore-crystal mixture of nematicidal Bt . Two (BC and BD) of the 6 isolates were selected based on their highest nematicidal activity against J2 of M. incognita with LC 50 values of 0.12 and 0.23 μg/ml of protein. The spore-crystal mixtures of isolates BC and BD when applied to tomato plants under greenhouse conditions exhibited the enhanced biocontrol potential by suppressing number of egg masses, reduction of female population and decreased root gall index, when compared to control and chemical treatments.
ISSN:2536-9342
1110-1768
2536-9342
DOI:10.1186/s41938-020-00293-2