Standardization of a latex agglutination test for coproantigen detection of Fasciola sp. in bovine cattle stool

Standardization of a latex agglutination test for coproantigen detection of Fasciola sp. in bovine cattle stool

Fasciolosis is a zoonotic parasitic disease, which affects humans and animals; diagnosed through noncommercial immunoassay tests that cannot be used on the field. Thereby, establishing the optimal conditions to develop a latex agglutination technique with IgG and IgM antibodies directed against excr...

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Bibliographic Details
Published in:Journal of parasitic diseases Vol. 42; no. 1; pp. 55 - 61
Main Authors: Orejarena Ávila, Lina Paola, Inguilan Benavides, Erika Marcela, Padilla Sanabria, Leonardo, Recalde-Reyes, Delia Piedad, Rodríguez-Salazar, Carlos Andrés, Castaño-Osorio, Jhon Carlos
Format: Journal Article
Language:English
Published: New Delhi Springer India 01-03-2018
Springer Nature B.V
Subjects:
Antigens
Bovidae
Cattle
Cryptosporidium
Enzyme-linked immunosorbent assay
Excretion
Fasciola
Giardia
Health Promotion and Disease Prevention
Immunoglobulin G
Immunoglobulin M
Immunoglobulins
Infectious Diseases
Latex agglutination
Medicine
Medicine & Public Health
Monoclonal antibodies
Original
Original Article
Protozoa
Standardization
Zoonoses
Monoclonal antibodies
Antigens
Agglutination
Fasciolosis
Latex
ELISA
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Summary:Fasciolosis is a zoonotic parasitic disease, which affects humans and animals; diagnosed through noncommercial immunoassay tests that cannot be used on the field. Thereby, establishing the optimal conditions to develop a latex agglutination technique with IgG and IgM antibodies directed against excretion/secretion antigens of Fasciola sp. is a priority. Latex particles were sensitized with IgG and IgM antibodies directed against excretion/secretion antigens of Fasciola sp. The specificity of the antibodies was determined against antigens of different helminths and protozoa; the sensitivity and specificity of the test was evaluated against a previously standardized direct ELISA. The coupling rates of the IgG and IgM antibodies were 85.77 and 100%, respectively. The minimum detectable concentration of Fasciola sp. excretion/secretion antigens, diluted in a phosphate-buffered saline, was 1.589 mg/mL(IgG) and 0.158 mg/mL(IgM) and for the antigens incorporated in the bovine cattle stool it was 3.178 mg/mL(IgG) and 1.589 mg/mL(IgM). The test showed crossed reaction against Giardia sp., and Cryptosporidium sp. antigens. Agreement of the IgG and IgM latex test against the ELISA test was of 78.78 and 96.96%, respectively; the specificity found was of 100% for both tests and sensitivity was 78.79% (IgG) and 96.97% (IgM). This work standardized the latex agglutination technique to detect Fasciola sp. antigens in bovine cattle stool.
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ISSN:0971-7196
0975-0703
DOI:10.1007/s12639-017-0964-9