CD44H Participates in the Intrahepatic Growth of Murine Colon 26 Adenocarcinoma Cells

The purpose of this study was to determine if CD44, a metastasis‐associated cell adhesion molecule, is involved in the hepatic colonization by murine colon 26 adenocarcinoma cells. Indirect membrane immunofluorescence and FACS analysis showed strong expressions of CD44 and integrin β1 on colon 26 ce...

Full description

Saved in:

Bibliographic Details
Published in:	Cancer science Vol. 89; no. 11; pp. 1160 - 1168
Main Authors:	Ogoshi, Takahiko, Ishii, Seiichi, Mizoi, Takayuki, Harada, Nobuhiko, Sato, Wataru, Saito, Kazuya, Ogawa, Hitoshi, Inabe, Keiko, Shiiba, Ken‐ichi, Matsuno, Seiki
Format:	Journal Article
Language:	English
Published:	Oxford, UK Blackwell Publishing Ltd 01-11-1998 Japanese Cancer Association John Wiley & Sons, Inc
Subjects:	Adenocarcinoma Adenocarcinoma - immunology Adenocarcinoma - pathology Analysis of Variance Animals Antibodies, Monoclonal Biological and medical sciences CD44 CD44 antigen Cell adhesion & migration Cell adhesion molecules Colon Colon 26 Colonization Colorectal cancer Colorectal Neoplasms - immunology Colorectal Neoplasms - pathology Epithelium Exons Flow cytometry Gastroenterology. Liver. Pancreas. Abdomen Hematopoiesis - immunology Hepatic metastasis Hyaluronan Receptors - immunology Immunofluorescence Inoculation Integrin beta1 - analysis Isoforms Liver Liver Neoplasms, Experimental - immunology Liver Neoplasms, Experimental - secondary Liver. Biliary tract. Portal circulation. Exocrine pancreas Male Medical sciences Metastases Metastasis Mice Mice, Inbred BALB C Monoclonal antibodies Nodules Polymerase chain reaction Protein Isoforms - immunology Reverse Transcriptase Polymerase Chain Reaction Reverse transcription Splenocytes Stomach. Duodenum. Small intestine. Colon. Rectum. Anus Tumor cells Tumor Cells, Cultured Tumors Animal model Pathophysiology Liver Rodentia Hepatic disease Metastasis Malignant tumor Experimental study In vitro Colonic disease Vertebrata Mammalia Guinea pig Animal Digestive diseases Intestinal disease Colon
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	The purpose of this study was to determine if CD44, a metastasis‐associated cell adhesion molecule, is involved in the hepatic colonization by murine colon 26 adenocarcinoma cells. Indirect membrane immunofluorescence and FACS analysis showed strong expressions of CD44 and integrin β1 on colon 26 cells. Injection of 1×105 colon 26 cells into the superior mesenteric vein of syngeneic BALB/c mice produced macroscopic hepatic nodules in 92% (22/24) of the mice 14 days after inoculation. When colon 26 cells were pretreated with an anti‐CD44 monoclonal antibody (mAb), IM7, only 30% (3/10) of the mice produced minute nodules in the liver on day 14 (P<0.001), though IM7 did not inhibit growth of the cells in vitro. Pretreatment of colon 26 cells with an anti‐integrin β1 mAb did not significantly block the hepatic metastasis. Histologically, microcolonies of tumor cells were detected in all of the livers on day 14 including the IM7‐pretreatment mice that were free of gross nodules. However, percentages of tumor‐occupied areas in the liver were consistently lower in IM7‐pretreatment mice than in control mice (0.82% vs. 5.0% on day 14; P<0.005). Reverse transcription‐polymerase chain reaction (RT‐PCR) amplification of mRNA revealed that colon 26 cells and splenocytes only expressed the hematopoietic isoform of CD44 (CD44H), which had no insertion of variant exons, while normal colonocytes expressed possible variant isoforms. These data suggest that malignant transformation of murine colonic epithelium altered the expression pattern of CD44 isoforms and that CD44H participates in the intrahepatic growth of colon 26 cells.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0910-5050 1347-9032 1349-7006 1876-4673
DOI:	10.1111/j.1349-7006.1998.tb00511.x