Search Results - "Impano, Stella"

The Elusive 5′-Deoxyadenosyl Radical: Captured and Characterized by Electron Paramagnetic Resonance and Electron Nuclear Double Resonance Spectroscopies by Yang, Hao, McDaniel, Elizabeth C, Impano, Stella, Byer, Amanda S, Jodts, Richard J, Yokoyama, Kenichi, Broderick, William E, Broderick, Joan B, Hoffman, Brian M

“…The 5′-deoxyadenosyl radical (5′-dAdo·) abstracts a substrate H atom as the first step in radical-based transformations catalyzed by…”
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S -Adenosyl-l-ethionine is a Catalytically Competent Analog of S -Adenosyl-l-methione (SAM) in the Radical SAM Enzyme HydG by Impano, Stella, Yang, Hao, Shepard, Eric M, Swimley, Ryan, Pagnier, Adrien, Broderick, William E, Hoffman, Brian M, Broderick, Joan B

“…Radical S-adenosyl-l-methionine (SAM) enzymes initiate biological radical reactions with the 5'-deoxyadenosyl radical (5'-dAdo•). A [4Fe-4S] cluster…”
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S -Adenosyl- l -ethionine is a Catalytically Competent Analog of S -Adenosyl- l -methionine (SAM) in the Radical SAM Enzyme HydG by Impano, Stella, Yang, Hao, Shepard, Eric M., Swimley, Ryan, Pagnier, Adrien, Broderick, William E., Hoffman, Brian M., Broderick, Joan B.

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Photoinduced Electron Transfer in a Radical SAM Enzyme Generates an S‑Adenosylmethionine Derived Methyl Radical by Yang, Hao, Impano, Stella, Shepard, Eric M, James, Christopher D, Broderick, William E, Broderick, Joan B, Hoffman, Brian M

“…Radical SAM (RS) enzymes use S-adenosyl-l-methionine (SAM) and a [4Fe–4S] cluster to initiate a broad spectrum of radical transformations throughout all…”
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Cover Picture: [FeFe]‐Hydrogenase: Defined Lysate‐Free Maturation Reveals a Key Role for Lipoyl‐H‐Protein in DTMA Ligand Biosynthesis (Angew. Chem. Int. Ed. 22/2022) by Pagnier, Adrien, Balci, Batuhan, Shepard, Eric M., Yang, Hao, Warui, Douglas M., Impano, Stella, Booker, Squire J., Hoffman, Brian M., Broderick, William E., Broderick, Joan B.

“…The generation of an active [FeFe]‐hydrogenase , the most efficient enzyme for H2 production, requires the synthesis of a complex metal center, the H‐cluster,…”
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S‐Adenosyl‐l‐ethionine is a Catalytically Competent Analog of S‐Adenosyl‐l‐methionine (SAM) in the Radical SAM Enzyme HydG by Impano, Stella, Yang, Hao, Shepard, Eric M., Swimley, Ryan, Pagnier, Adrien, Broderick, William E., Hoffman, Brian M., Broderick, Joan B.

“…Radical S‐adenosyl‐l‐methionine (SAM) enzymes initiate biological radical reactions with the 5′‐deoxyadenosyl radical (5′‐dAdo.). A [4Fe‐4S]+ cluster…”
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[FeFe]-Hydrogenase: Defined Lysate-Free Maturation Reveals a Key Role for Lipoyl-H-Protein in DTMA Ligand Biosynthesis by Pagnier, Adrien, Balci, Batuhan, Shepard, Eric M., Yang, Hao, Warui, Douglas M., Impano, Stella, Booker, Squire J., Hoffman, Brian M., Broderick, William E., Broderick, Joan B.

“…Abstract Maturation of [FeFe]‐hydrogenase (HydA) involves synthesis of a CO, CN − , and dithiomethylamine (DTMA)‐coordinated 2Fe subcluster that is inserted…”
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Paradigm Shift for Radical S‑Adenosyl‑l‑methionine Reactions: The Organometallic Intermediate Ω Is Central to Catalysis by Byer, Amanda S, Yang, Hao, McDaniel, Elizabeth C, Kathiresan, Venkatesan, Impano, Stella, Pagnier, Adrien, Watts, Hope, Denler, Carly, Vagstad, Anna L, Piel, Jörn, Duschene, Kaitlin S, Shepard, Eric M, Shields, Thomas P, Scott, Lincoln G, Lilla, Edward A, Yokoyama, Kenichi, Broderick, William E, Hoffman, Brian M, Broderick, Joan B

“…Radical S-adenosyl-l-methionine (SAM) enzymes comprise a vast superfamily catalyzing diverse reactions essential to all life through homolytic SAM cleavage to…”
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[FeFe]‐Hydrogenase: Defined Lysate‐Free Maturation Reveals a Key Role for Lipoyl‐H‐Protein in DTMA Ligand Biosynthesis by Pagnier, Adrien, Balci, Batuhan, Shepard, Eric M., Yang, Hao, Warui, Douglas M., Impano, Stella, Booker, Squire J., Hoffman, Brian M., Broderick, William E., Broderick, Joan B.

“…Maturation of [FeFe]‐hydrogenase (HydA) involves synthesis of a CO, CN−, and dithiomethylamine (DTMA)‐coordinated 2Fe subcluster that is inserted into HydA to…”
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New Insights into Radical Initiation by Radical S-Adenosylmethionine Enzymes and Activation of [FeFe]-Hydrogenase by Impano, Stella

“…Radical S-adenosylmethionine (SAM) enzymes harbor a [4Fe-4S] cluster in their active sites that coordinates a catalytically relevant small molecule SAM. During…”
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Active-Site Controlled, Jahn–Teller Enabled Regioselectivity in Reductive S–C Bond Cleavage of S -Adenosylmethionine in Radical SAM Enzymes by Impano, Stella, Yang, Hao, Jodts, Richard J., Pagnier, Adrien, Swimley, Ryan, McDaniel, Elizabeth C., Shepard, Eric M., Broderick, William E., Broderick, Joan B., Hoffman, Brian M.

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Mechanistic Studies of Radical SAM Enzymes: Pyruvate Formate-Lyase Activating Enzyme and Lysine 2,3-Aminomutase Case Studies by Byer, Amanda S, McDaniel, Elizabeth C, Impano, Stella, Broderick, William E, Broderick, Joan B

“…The radical SAM enzyme superfamily is large and diverse, with ever-increasing numbers of examples of characterized reactions. This chapter focuses on the…”
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Active-Site Controlled, Jahn-Teller Enabled Regioselectivity in Reductive S-C Bond Cleavage of S -Adenosylmethionine in Radical SAM Enzymes by Impano, Stella, Yang, Hao, Jodts, Richard J, Pagnier, Adrien, Swimley, Ryan, McDaniel, Elizabeth C, Shepard, Eric M, Broderick, William E, Broderick, Joan B, Hoffman, Brian M

“…Catalysis by canonical radical -adenosyl-l-methionine (SAM) enzymes involves electron transfer (ET) from [4Fe-4S] to SAM, generating an R S radical that…”
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Radical SAM Enzyme Spore Photoproduct Lyase: Properties of the Ω Organometallic Intermediate and Identification of Stable Protein Radicals Formed during Substrate-Free Turnover by Pagnier, Adrien, Yang, Hao, Jodts, Richard J, James, Christopher D, Shepard, Eric M, Impano, Stella, Broderick, William E, Hoffman, Brian M, Broderick, Joan B

“…Spore photoproduct lyase is a radical S-adenosyl-l-methionine (SAM) enzyme with the unusual property that addition of SAM to the [4Fe-4S]1+ enzyme absent…”
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HydG, the "dangler" iron, and catalytic production of free CO and CN - : implications for [FeFe]-hydrogenase maturation by Shepard, Eric M, Impano, Stella, Duffus, Benjamin R, Pagnier, Adrien, Duschene, Kaitlin S, Betz, Jeremiah N, Byer, Amanda S, Galambas, Amanda, McDaniel, Elizabeth C, Watts, Hope, McGlynn, Shawn E, Peters, John W, Broderick, William E, Broderick, Joan B

“…The organometallic H-cluster of the [FeFe]-hydrogenase consists of a [4Fe-4S] cubane bridged via a cysteinyl thiolate to a 2Fe subcluster ([2Fe]H) containing…”
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[FeFe]‐Hydrogenase: Defined Lysate‐Free Maturation Reveals a Key Role for Lipoyl‐H‐Protein in DTMA Ligand Biosynthesis by Pagnier, Adrien, Balci, Batuhan, Shepard, Eric M., Yang, Hao, Warui, Douglas M., Impano, Stella, Booker, Squire J., Hoffman, Brian M., Broderick, William E., Broderick, Joan B.

“…Maturation of [FeFe]‐hydrogenase (HydA) involves synthesis of a CO, CN−, and dithiomethylamine (DTMA)‐coordinated 2Fe subcluster that is inserted into HydA to…”
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Titelbild: [FeFe]‐Hydrogenase: Defined Lysate‐Free Maturation Reveals a Key Role for Lipoyl‐H‐Protein in DTMA Ligand Biosynthesis (Angew. Chem. 22/2022) by Pagnier, Adrien, Balci, Batuhan, Shepard, Eric M., Yang, Hao, Warui, Douglas M., Impano, Stella, Booker, Squire J., Hoffman, Brian M., Broderick, William E., Broderick, Joan B.

“…Die Bildung einer aktiven [FeFe]‐Hydrogenase , des effizientesten Enzyms für die H2‐Produktion, erfordert die Synthese eines komplexen Metallzentrums, des…”
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S‐Adenosyl‐l‐ethionine is a Catalytically Competent Analog of S‐Adenosyl‐l‐methionine (SAM) in the Radical SAM Enzyme HydG by Impano, Stella, Yang, Hao, Shepard, Eric M., Swimley, Ryan, Pagnier, Adrien, Broderick, William E., Hoffman, Brian M., Broderick, Joan B.

“…Radical S‐adenosyl‐l‐methionine (SAM) enzymes initiate biological radical reactions with the 5′‐deoxyadenosyl radical (5′‐dAdo.). A [4Fe‐4S]+ cluster…”
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Titelbild: [FeFe]‐Hydrogenase: Defined Lysate‐Free Maturation Reveals a Key Role for Lipoyl‐H‐Protein in DTMA Ligand Biosynthesis (Angew. Chem. 22/2022) by Pagnier, Adrien, Balci, Batuhan, Shepard, Eric M., Yang, Hao, Warui, Douglas M., Impano, Stella, Booker, Squire J., Hoffman, Brian M., Broderick, William E., Broderick, Joan B.

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