Observation of triple helix motif on electrospun collagen nanofibers and its effect on the physical and structural properties

Collagen is a very popular natural biomaterial due to its high biocompatibility and bioactivity. Electrospinning is currently the only technique that allows the fabrication of continuous fibers with diameters down to a few nanometers. In order to regenerate collagen in the forms of nanofibers, it is...

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Bibliographic Details
Published in:	Journal of molecular structure Vol. 1151; pp. 73 - 80
Main Authors:	Bürck, Jochen, Aras, Onur, Bertinetti, Luca, Ilhan, Caner A., Ermeydan, Mahmut A., Schneider, Reinhard, Ulrich, Anne S., Kazanci, Murat
Format:	Journal Article
Language:	English
Published:	Elsevier B.V 05-01-2018
Subjects:	3D native structure Collagen Electrospinning SEM TEM Electrospinning SEM 3D native structure TEM Collagen
Online Access:	Get full text
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Summary:	Collagen is a very popular natural biomaterial due to its high biocompatibility and bioactivity. Electrospinning is currently the only technique that allows the fabrication of continuous fibers with diameters down to a few nanometers. In order to regenerate collagen in the forms of nanofibers, it is necessary to dissolve it in suitable solvents. The solvents and electrospinning process cause unfolding of collagen nanofibers. It is proposed that acidic solvents preserve better the natural structure of collagen fibers. In this paper, the structures of collagen nanofibers were examined by using circular dichroism (CD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Raman spectroscopy, differential scanning calorimetry (DSC) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) methods in order to test this hypothesis. The increase in PP-II fraction, representing the triple helix structure in collagen, that was observed in CD analysis of HAc derived collagen nanofibers, for the first time was successfully confirmed and illustrated by using SEM and TEM methods. Furthermore, CD revealed the mostly detrimental effect of stabilization conditions such as heat, vacuum and UV treatment on the secondary structure of the collagen nanofibers. •The partially preserved triple helix structure in HAc derived collagen nanofibers was observed.•The partial preservation of the native structure does not effect that much the overall fiber properties.•Cd results revealed that the crosslinking methods are mostly detrimental to the native structure of collagen.
ISSN:	0022-2860 1872-8014
DOI:	10.1016/j.molstruc.2017.09.030