Clustering of Staphylococcus aureus bovine mastitis strains from regions of Central-Eastern Poland based on their biochemical and genetic characteristics

Clustering of Staphylococcus aureus bovine mastitis strains from regions of Central-Eastern Poland based on their biochemical and genetic characteristics

Staphylococcus aureus strains were isolated from mastitic milk of cows with infected mammary glands. The animals were living in 12 different farms near Lublin, in Central-Eastern Poland. A biochemical identification method based on enzymatic assay was performed, followed by haemolytic and proteolyti...

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Bibliographic Details
Published in:Polish journal of veterinary sciences Vol. 18; no. 2; pp. 333 - 342
Main Authors: Puacz, E, Ilczyszyn, W M, Kosecka, M, Buda, A, Dudziak, W, Polakowska, K, Panz, T, Białecka, A, Kasprowicz, A, Lisowski, A, Krukowski, H, Cuteri, V, Międzobrodzki, J
Format: Journal Article
Language:English
Published: Germany Polish Academy of Sciences, Committee of Veterinary Sciences 01-06-2015
Subjects:
Animals
Cattle
Drug Resistance, Bacterial - genetics
Female
Mastitis, Bovine - epidemiology
Mastitis, Bovine - microbiology
Oligonucleotide Array Sequence Analysis
Phylogeny
Poland - epidemiology
Staphylococcal Infections - microbiology
Staphylococcal Infections - veterinary
Staphylococcus aureus - classification
Staphylococcus aureus - genetics
Staphylococcus aureus - physiology
Virulence - genetics
Poland
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Summary:Staphylococcus aureus strains were isolated from mastitic milk of cows with infected mammary glands. The animals were living in 12 different farms near Lublin, in Central-Eastern Poland. A biochemical identification method based on enzymatic assay was performed, followed by haemolytic and proteolytic tests. PCR-RFLP targeted on the gap gene allowed the genetic identification of strains at the species level and verified phenotypic identification results. A molecular typing method using triplex PCR was performed to recognize the genetic similarity of the analyzed strains. DNA microarray hybridization (StaphyType, Alere Technologies) was used for detection of antibiotic resistance and virulence associated markers. The results obtained indicate high genetic similarity in strains isolated from the same sites. High genetic similarities were also detected between strains isolated from cows from different farms of the same region. A slightly lower similarity was noted however, in strains from various regions indicating that the strains are herd specific and that the cow's infections caused by S. aureus were of a clonal character. In 21 representative isolates selected for DNA-microarray testing, only fosfomycin (fosB) and penicillin resistance markers (blaZ, blaI, blaR) were detected. The presence of genes coding for haemolysins (lukF, lukS, hlgA, hla, hld, hlb), proteases (aur, sspA, sspB, sspP), enterotoxins (entA, entD, entG, entI, entJ, entM, entN, entO, entR, entU, egc-cluster), adhesins (icaA, icaC, icaD, bbp, clfA, clfB, fib, fnbA, map, vwb) or immune evasion proteins (scn, chp, sak) was common and, with exceptions, matched triplex PCR-defined clusters.
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ISSN:1505-1773
2300-2557
DOI:10.1515/pjvs-2015-0043