Evaluation of induced spawning on oocyte characteristics and serum biochemistry of African catfish (Clarias gariepinus)

Evaluation of induced spawning on oocyte characteristics and serum biochemistry of African catfish (Clarias gariepinus)

The effects of induced spawning using salmon gonadotropin-releasing hormone analogue (GnRHa) and domperidone proprietary drug (ovaprim) injected (0.4 ml/kg) intramuscularly on oocyte characteristics, sex hormones, thyroid hormones, and serum biochemical response were evaluated at 0, 12, 24, and 36 h...

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Bibliographic Details
Published in:Comparative clinical pathology Vol. 29; no. 1; pp. 103 - 114
Main Authors: Okoye, Chidozie Nwabuisi, Dan-Jumbo, Susan Omoboba, Emejue, Calistus Onyedika, Udoumoh, Anietie Francis, Agina, Onyinyechukwu Ada, Uju, Chinelo Nnenna, Ochiogu, Izuchukwu Shedrack, Onuorah, Ifeoma J., Obidike, Ikechukwu Reginald, Ihedioha, John Ikehchukwu
Format: Journal Article
Language:English
Published: London Springer London 01-02-2020
Springer Nature B.V
Subjects:
Calcium
Cholesterol
Clarias gariepinus
Estrogens
Gonadotropin-releasing hormone
Gonadotropins
Hematology
Hormones
Medicine
Medicine & Public Health
Oncology
Oocytes
Original Article
Pathology
Pituitary (anterior)
Salmon
Serum levels
Sex hormones
Sodium
Spawning
Testosterone
Thyroid hormones
Triiodothyronine
Testosterone
Thyroid hormones
Serum biochemistry
Spawning
Oocyte
Estrogen
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Summary:The effects of induced spawning using salmon gonadotropin-releasing hormone analogue (GnRHa) and domperidone proprietary drug (ovaprim) injected (0.4 ml/kg) intramuscularly on oocyte characteristics, sex hormones, thyroid hormones, and serum biochemical response were evaluated at 0, 12, 24, and 36 h post-induction. Twenty-eight 8 months old female Clarias gariepinus were used. At the designated hour, 7 of these fish were randomly selected for sampling. The response to the induction of spawning using ovaprim was time-dependent. The onset of response was observed from 12 h for increase in GSI, oocyte flotation, and oocyte diameter. The mean diameter of the different categories of the oocytes was significantly higher than those of the control at 24 h post-induction. Spawning was associated with a significant increase in serum estrogen and calcium level from 12 h post-induction. Furthermore, induction of spawning resulted in significantly higher serum levels of cholesterol, testosterone, and triiodothyronine as well as significantly higher activities of ALT and AST at different time-points. However, the BUN and serum levels of chloride and sodium were significantly lower compared with the control. From this study, the time for stripping of roe for optimal spawning outcome may be between 12 and 24 h.
ISSN:1618-5641
1618-565X
DOI:10.1007/s00580-019-03038-2