The value of anal swab RT-PCR for COVID-19 diagnosis in adult Indonesian patients

ObjectiveThis study will test the performance of the anal swab PCR test when compared with the nasopharyngeal swab PCR test as a diagnostic tool for COVID-19.DesignAn observational descriptive study which included hospitalised suspected, or probable cases of hopitalised COVID-19 patients, conducted...

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Published in:	BMJ open gastroenterology Vol. 8; no. 1; p. e000590
Main Authors:	Abdullah, Murdani, Sudrajat, Dedy Gunawanjati, Muzellina, Virly Nanda, Kurniawan, Juferdy, Rizka, Aulia, Utari, Amanda Pitarini, Pribadi, Rabbinu Rangga, Idrus, Muhammad Firhat, Yusra, Yusra, Meilany, Sofy, Surandy, Andry, Shatri, Hamzah, Rinaldi, Ikhwan, Pitoyo, Ceva Wicaksono, Renaldi, Kaka
Format:	Journal Article
Language:	English
Published:	England BMJ Publishing Group LTD 19-05-2021 BMJ Publishing Group
Subjects:	Biopsy Body mass index Comorbidity Coronaviruses COVID-19 Diarrhea Disease transmission Infection Infections Medical diagnosis Mortality Patients Pneumonia Respiratory distress syndrome Severe acute respiratory syndrome coronavirus 2 Small intestine Indonesia COVID-19 anal diagnostic virology
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Summary:	ObjectiveThis study will test the performance of the anal swab PCR test when compared with the nasopharyngeal swab PCR test as a diagnostic tool for COVID-19.DesignAn observational descriptive study which included hospitalised suspected, or probable cases of hopitalised COVID-19 patients, conducted in Dr. Cipto Mangunkusumo National Hospital, Ciputra Hospital, Mitra Keluarga Depok Hospital and Mitra Keluarga Kelapa Gading Hospital, Indonesia. Epidemiological, clinical, laboratory and radiology data were obtained. Nasopharyngeal and anal swabs specimens were collected for SARS-CoV-2 RNA detection.ResultsWe analysed 136 subjects as part of this study. The clinical spectrum of COVID-19 manifesation in this study was typical of hospitalised patients, with 25% classified as mild cases, 14.7% in severe condition and 12.5% of subjects classified as having acute respiratory distress syndrome. When compared with nasopharyngeal swab as the standard specimen for reverse transcription polymerase chain reaction (RT-PCR) detection of SARS-CoV-2 antigen, the sensitivity and specificity of the anal swab was 36.7% and 93.8%, respectively. The positive and negative predictive value were 97.8% and 16.5 %, respectively. The performance of the anal swab remained similar when only the subgroup of patients with gastrointestinal symptoms (n=92, 67.6%) was analysed (sensitivity 40% and specificity 91.7%). Out of all the subjects included in analysis, 67.6% had gastrointestinal symptoms. Similarly, 73.3% of patients in the anal swab-positive group had gastrointestinal symptoms. The two most common gastrointestinal symptoms in the subjects’ population were nausea and anorexia.ConclusionAnal swab specimen has low sensitivity (36.7%) but high specificity (93.8%) for detecting SARS-CoV-2 antigen by RT-PCR. Only one additional positive result was found by anal swab among the nasopharyngeal swab-negative group. Anal swab may not be needed as an additional test at the beginning of a patient’s diagnostic investigation and nasopharyngeal swab RT-PCR remains as the standard diagnostic test for COVID-19.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Undefined-1 ObjectType-Feature-3 content type line 23
ISSN:	2054-4774 2054-4774
DOI:	10.1136/bmjgast-2020-000590