A transgenic bacterial artificial chromosome approach to identify regulatory regions that direct Amhr2 and Osterix expression in Müllerian duct mesenchyme
A transgenic mouse approach using bacterial artificial chromosomes (BAC) was used to identify regulatory regions that direct Müllerian duct expression for and ( , also known as ). encodes the receptor that mediates anti-Müllerian hormone (AMH) signaling for Müllerian duct regression in male embryos....
Saved in:
| Published in: | Frontiers in cell and developmental biology Vol. 10; p. 1006087 |
|---|---|
| Main Authors: | , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Switzerland
Frontiers media
12-10-2022
Frontiers Media S.A |
| Subjects: | |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | A transgenic mouse approach using bacterial artificial chromosomes (BAC) was used to identify regulatory regions that direct Müllerian duct expression for
and
(
, also known as
).
encodes the receptor that mediates anti-Müllerian hormone (AMH) signaling for Müllerian duct regression in male embryos.
is expressed in the Müllerian duct mesenchyme of both male and female embryos. A ∼147-kb BAC clone containing the
locus was used to generate transgenic mice. The transgene was able to rescue the block in Müllerian duct regression of
-null males, suggesting that the BAC clone contains regulatory sequences active in male embryos.
is expressed in the developing skeleton of male and female embryos but is also an AMH-induced gene that is expressed in the Müllerian duct mesenchyme exclusively in male embryos.
transgenic mice were previously generated using a ∼204-kb BAC clone. Crosses of
mice to Cre-dependent
reporter mice resulted in reporter expression in the developing skeleton and in the Müllerian duct mesenchyme of male but not female embryos.
transgenic mice were previously generated using a 39-kb genomic region surrounding the
locus.
embryos expressed red fluorescence in the developing skeleton and Müllerian duct mesenchyme of male but not female embryos. In addition, female
embryos ectopically expressing human AMH from an
transgene activated red fluorescence in the Müllerian duct mesenchyme. These results suggest that the 39-kb region used to generate
contains male-specific Müllerian duct mesenchyme regulatory sequences that are responsive to AMH signaling. These BAC transgenic mouse approaches identify two distinct regions that direct Müllerian duct mesenchyme expression and contribute fundamental knowledge to define a gene regulatory network for sex differentiation. |
|---|---|
| Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 PMCID: PMC9597298 This article was submitted to Molecular and Cellular Reproduction, a section of the journal Frontiers in Cell and Developmental Biology These authors have contributed equally to this work and share senior authorship Edited by: Fei Gao, Institute of Zoology (CAS), China Isabelle Stévant, Bar-Ilan University, Israel Blanche Capel, Duke University Medical Center, United States Francis Poulat, Institut National de la Santé et de la Recherche Médicale (INSERM), France Reviewed by: Yi Athena Ren, Cornell University, United States Nitzan Gonen, Bar-Ilan University, Israel |
| ISSN: | 2296-634X 2296-634X |
| DOI: | 10.3389/fcell.2022.1006087 |