Oncolytic attenuated measles virus encoding NY-ESO-1 induces HLA I and II presentation of this tumor antigen by melanoma and dendritic cells

Antitumor virotherapy stimulates the antitumor immune response during tumor cell lysis induced by oncolytic viruses (OVs). OV can be modified to express additional transgenes that enhance their therapeutic potential. In this study, we armed the spontaneously oncolytic Schwarz strain of measles virus...

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Bibliographic Details
Published in:	Cancer Immunology, Immunotherapy Vol. 72; no. 10; pp. 3309 - 3322
Main Authors:	Grard, Marion, Idjellidaine, Mohamed, Arbabian, Atousa, Chatelain, Camille, Berland, Laurine, Combredet, Chantal, Dutoit, Soizic, Deshayes, Sophie, Dehame, Virginie, Labarrière, Nathalie, Fradin, Delphine, Boisgerault, Nicolas, Blanquart, Christophe, Tangy, Frédéric, Fonteneau, Jean-François
Format:	Journal Article
Language:	English
Published:	Berlin/Heidelberg Springer Berlin Heidelberg 01-10-2023 Springer Nature B.V Springer Verlag
Subjects:	Antibodies - metabolism Antigen presentation Antigens Antigens, Neoplasm Antitumor activity Cancer Research CD4 antigen CD8 antigen CD8-Positive T-Lymphocytes Dendritic Cells Humans Immunology Life Sciences Lymphocytes T Lysis Male Measles Measles - metabolism Measles virus - genetics Medicine Medicine & Public Health Melanoma Melanoma - metabolism Membrane Proteins Monocytes NY-ESO-1 protein Oncology Oncolysis Oncolytic Viruses - genetics Transgenes Tumor cells Tumors Viruses NY-ESO-1 Antitumor immunotherapy T cells Tumor antigen Melanoma Measles virus tumor antigen melanoma measles virus antitumor immunotherapy
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Summary:	Antitumor virotherapy stimulates the antitumor immune response during tumor cell lysis induced by oncolytic viruses (OVs). OV can be modified to express additional transgenes that enhance their therapeutic potential. In this study, we armed the spontaneously oncolytic Schwarz strain of measles viruses (MVs) with the gene encoding the cancer/testis antigen NY-ESO-1 to obtain MVny. We compared MV and MVny oncolytic activity and ability to induce NY-ESO-1 expression in six human melanoma cell lines. After MVny infection, we measured the capacity of melanoma cells to present NY-ESO-1 peptides to CD4 + and CD8 + T cell clones specific for this antigen. We assessed the ability of MVny to induce NY-ESO-1 expression and presentation in monocyte-derived dendritic cells (DCs). Our results show that MVny and MV oncolytic activity are similar with a faster cell lysis induced by MVny. We also observed that melanoma cell lines and DC expressed the NY-ESO-1 protein after MVny infection. In addition, MVny-infected melanoma cells and DCs were able to stimulate NY-ESO-1-specific CD4 + and CD8 + T cells. Finally, MVny was able to induce DC maturation. Altogether, these results show that MVny could be an interesting candidate to stimulate NY-ESO-1-specific T cells in melanoma patients with NY-ESO-1-expressing tumor cells.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 PMCID: PMC10992919
ISSN:	0340-7004 1432-0851 1432-0851
DOI:	10.1007/s00262-023-03486-4