Transglycosylation activity of β- N-acetylhexosaminidase from Penicillium oxalicum and its application to synthesis of a drug carrier

Penicillium oxalicum β- N-acetylhexosaminidase was found to have transglycosylation activity in addition to hydrolytic activity. The enzyme effectively transferred N-acetylglucosamine to various alcohols in the presence of N-acetylchitooligomers as donors. The enzyme also transferred N-acetylglucosa...

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Bibliographic Details
Published in:	Journal of fermentation and bioengineering Vol. 83; no. 4; pp. 341 - 345
Main Authors:	Kadowaki, Setsu, Saskiawan, Iwan, Watanabe, Jun, Yamamoto, Kenji, Bunno, Masayasu, Ichihara, Yoshihiro, Kumagai, Hidehiko
Format:	Journal Article
Language:	English
Published:	Osaka Elsevier B.V 01-01-1997 Society for Fermentation and Bioengineering
Subjects:	Biological and medical sciences Biotechnology drug carrier DRUGS Fundamental and applied biological sciences. Psychology Health. Pharmaceutical industry HIDROLASAS HYDROLASE HYDROLASES Industrial applications and implications. Economical aspects MEDICAMENT MEDICAMENTOS Miscellaneous N-acetylchitooligomer PENICILLIUM OXALICUM transglycosylation β- N-acetylhexosaminidase Penicillium oxalicum transglycosylation N-acetylchitooligomer drug carrier β- N-acetylhexosaminidase Enzymatic synthesis Enzyme β-N-Acetylhexosaminidase Drug carrier Fungi Transglycosylation Glycosidases Aminoglycoside Hydrolases Fungi Imperfecti O-Glycosidases Thallophyta Enzymatic reaction
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Summary:	Penicillium oxalicum β- N-acetylhexosaminidase was found to have transglycosylation activity in addition to hydrolytic activity. The enzyme effectively transferred N-acetylglucosamine to various alcohols in the presence of N-acetylchitooligomers as donors. The enzyme also transferred N-acetylglucosamine to diol-derivatives. Using this transglycosylation activity, a drug carrier, 6-benzyloxyhexyl-β- N-acetylglucosaminide, was synthesized by incubating the enzyme with 6-benzyloxyhexan-1-ol and N-acetylchitotriose. The product was isolated by silica gel column chromatography and was analyzed by mass-spectrometry and 1H-NMR.
Bibliography:	F60 1997007153 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0922-338X
DOI:	10.1016/S0922-338X(97)80139-0