Rapid protocol of Aloe Vera in Vitro propagation
The current research was carried out to enhance Aloe vera propagation via tissue culture technique. BA at 0, 1, 2, 3, and 4 mg/l alone or combined with 0.1 mg/l NAA were used for shoot proliferation. The best result was recorded by using 2mgl-1 BAP (5.33 shoots/ explant). Meanwhile, combination betw...
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| Published in: | Iraqi journal of agricultural science Vol. 50; no. 5; pp. 1377 - 1382 |
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| Main Authors: | , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Baghdad, Iraq
University of Baghdad, College of Agriculture
23-10-2019
Baghdad University |
| Subjects: | |
| Online Access: | Get full text |
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| Summary: | The current research was carried out to enhance Aloe vera propagation via tissue culture technique. BA at 0, 1, 2, 3, and 4 mg/l alone or combined with 0.1 mg/l NAA were used for shoot proliferation. The best result was recorded by using 2mgl-1 BAP (5.33 shoots/ explant). Meanwhile, combination between 2 mg/l BAP with (0.2, 0.4 and 0.6) mg/l kinetin, NAA or IBA were tested and best protocol was shown by using 2 mg1-1 BAP + 0.6 NAA which recorded 4.89 shoots/ explant. For rooting, MS medium at half and full strength salts were used supplemented with 0, 1, 2, 3, 4mgl-1 of NAA. The result revealed that 3 mg1-1 NAA at half strength of MS medium regenerate developed roots (8.67 roots / shoot) within 4 weeks. The well successful healthy plantlets were transferred into a potting mix composed of sand and peat moss which shows 100% survival ratio. |
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| ISSN: | 0075-0530 2410-0862 |
| DOI: | 10.36103/ijas.v50i5.804 |