A promoter derived from taro bacilliform badnavirus drives strong expression in transgenic banana and tobacco plants

Taro bacilliform virus (TaBV) is a pararetrovirus of the genus Badnavirus which infects the monocotyledonous plant, taro ( Colocasia esculenta). A region of the TaBV genome spanning nucleotides 6,281 to 12 (T1200), including the 3' end of open reading frame 3 (ORF 3) and the intergenic region t...

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Bibliographic Details
Published in:	Plant cell reports Vol. 21; no. 12; pp. 1199 - 1206
Main Authors:	YANG, I. C, IOMMARINI, J. P, BECKER, D. K, HAFNER, G. J, DALE, J. L, HARDING, R. M
Format:	Journal Article
Language:	English
Published:	Berlin Springer 01-08-2003 Springer Nature B.V
Subjects:	Agronomy. Soil science and plant productions Badnavirus - genetics Binding sites Biological and medical sciences Biotechnology Classical and quantitative genetics. Population genetics. Molecular genetics Cloning, Molecular Colocasia - virology Culture Techniques Fruits Fundamental and applied biological sciences. Psychology Gene Expression Regulation Generalities. Genetics. Plant material Genetic engineering Genetic technics Genetics and breeding of economic plants Leaves Methods. Procedures. Technologies Molecular and cellular biology Molecular genetics Musa - genetics Nicotiana - genetics Plant tissues Promoter Regions, Genetic Proteins Tobacco Transcription. Transcription factor. Splicing. Rna processing Transgenes Transgenic animals and transgenic plants Transgenic plants Taro bacilliform virus Cell culture Monocotyledones Callus Plant pathogen Transcription promoter Stimulant plant Nicotiana tabacum Caulimoviridae Stem Promoter Plant Badnavirus Dicotyledones Angiospermae Transgenic plant Solanaceae Conductive tissue Genetic transformation Root Tissue specificity Musaceae Plant leaf Gene expression Banana Virus Musa Tobacco Spermatophyta
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Summary:	Taro bacilliform virus (TaBV) is a pararetrovirus of the genus Badnavirus which infects the monocotyledonous plant, taro ( Colocasia esculenta). A region of the TaBV genome spanning nucleotides 6,281 to 12 (T1200), including the 3' end of open reading frame 3 (ORF 3) and the intergenic region to the end of the tRNA(met)-binding site, was tested for promoter activity along with four different 5' deletion fragments (T600, T500, T250 and T100). In transient assays, only the T1200, T600, T500 fragments were shown to have promoter activity in taro leaf, banana suspension cells and tobacco callus. When these three promoters were evaluated in stably transformed, in vitro-grown transgenic banana and tobacco plants, all were found to drive near-constitutive expression of either the green fluorescent protein or beta-glucuronidase (GUS) reporter gene in the stem (or pseudostem), leaves and roots, with strongest expression observed in the vascular tissue. In transgenic banana leaves, the T600 promoter directed four-fold greater GUS activity than that of the T1200, T500 and the maize polyubiquitin-1 promoters. In transgenic tobacco leaves, the levels of GUS expression directed by the three promoters was between four- and ten-fold lower than that of the double Cauliflower mosaic virus 35S promoter. These results indicate that the TaBV-derived promoters may be useful for the high-level constitutive expression of transgenes in either monocotyledonous or dicotyledonous species.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0721-7714 1432-203X
DOI:	10.1007/s00299-003-0621-x