The International Standard for Atrial Natriuretic Factor Calibration by an International Collaborative Study

An ampouled preparation of human atrial natriuretic factor, ANF-(99–126), was evaluated by 23 laboratories in 10 countries for its suitability to serve as the international standard for ANF. The preparation was calibrated by radfoimmunoassay, radioreceptor binding assay, and bioassay and was shown t...

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Bibliographic Details
Published in:	Hypertension (Dallas, Tex. 1979) Vol. 12; no. 6; pp. 629 - 634
Main Authors:	POOLE, STEPHEN, DAS, ROSE E. GAINES, DZAU, VICTOR J, ICHARDS, A MARK, IAN, J, ROBERTSON, S
Format:	Journal Article
Language:	English
Published:	Philadelphia, PA American Heart Association, Inc 01-12-1988 Hagerstown, MD Lippincott
Subjects:	Arterial hypertension. Arterial hypotension Atrial Natriuretic Factor - blood Atrial Natriuretic Factor - standards Biological and medical sciences Blood and lymphatic vessels Calibration Cardiology. Vascular system Clinical manifestations. Epidemiology. Investigative techniques. Etiology Drug Stability Humans Hydrogen-Ion Concentration Medical sciences Hypertension Arterial pressure Atrial natriuretic factor
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Summary:	An ampouled preparation of human atrial natriuretic factor, ANF-(99–126), was evaluated by 23 laboratories in 10 countries for its suitability to serve as the international standard for ANF. The preparation was calibrated by radfoimmunoassay, radioreceptor binding assay, and bioassay and was shown to have satisfactory stability and biological activity. Estimates of the ANF content of a set of specimens of plasma in terms of the standard showed agreement in ranking order when the ANF was extracted prior to assay. However, estimates of the ANF content of the plasmas in terms of either the international standard or the various local standards varied widely among laboratories. On the basis of the results reported here, with the agreement of the participants in the study and with the authorization of the Expert Committee on Biological Standardization of the World Health Organization, the preparation coded 85/669 was established in 1987 as the international standard for ANF, with a denned potency of 2.5 international units per ampoule.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0194-911X 1524-4563
DOI:	10.1161/01.HYP.12.6.629