Comparison of Cardiovascular Effects of Ribes Khorassanicum Fractions with Its Total Extract in Normotensive Rats
BACKGROUND AND OBJECTIVE: Ribes khorassanicum has beneficial cardiovascular effects in normotensive and hypertensive animals. For further investigation, the effect of three fractions of aqueous (polar), ethyl acetate (polar and non-polar) and n-hexane (non-polar) of the plant s hydroalcoholic extrac...
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| Published in: | Majallah-i Dānishgāh-i ʻUlūm-i Pizishkī̄-i Bābul Vol. 23; no. 1; pp. 9 - 15 |
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| Main Authors: | , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Babol University of Medical Sciences
01-03-2021
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| Subjects: | |
| Online Access: | Get full text |
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| Summary: | BACKGROUND AND OBJECTIVE: Ribes khorassanicum has beneficial cardiovascular effects in normotensive and hypertensive animals. For further investigation, the effect of three fractions of aqueous (polar), ethyl acetate (polar and non-polar) and n-hexane (non-polar) of the plant s hydroalcoholic extract on cardiovascular parameters in normotensive rats was compared with its total extract. METHODS: In this experimental study, 42 rats were divided into 7 groups (6 in each group): control, three doses of total extract 4, 12 and 24 mg/kg and three fractions of aqueous, ethyl acetate and n-hexane (20 mg/kg). Femoral artery cannulation and heart rate (HR), mean arterial pressure (MAP) and systolic blood pressure (SBP) were recorded throughout the experiment and then the changes (Δ: difference before and after injection) were calculated and statistically analyzed. FINDINGS: 24 mg/kg of the extract significantly increased ΔHR (117.5±11.6 beats per minute) compared to control (5.3±2.7, p<0.001) and 12 mg/kg of the extract significantly decreased ΔMAP (-15.1±2.8 mm Hg) and ΔSBP (-18.4±3 mm Hg) compared to the control (p<0.05). The fractions increased ΔHR, which was not significant but was significant compared to 24 mg/kg of the extract (p<0.05). Furthermore, the increase of ΔMAP and ΔSBP in n-hexane fraction (19.45±3.1 mm Hg and 34.6±11.5 mm Hg, respectively) was significant compared to the control and 12 mg/kg of the extract (p<0.01 to p<0.05). CONCLUSION: The active ingredients in the cardiovascular effects of Ribes khorassanicum extract are spread in all fractions and the total extract has a better effect than each of the fractions alone due to its various non-polar and polar compounds as well as the synergistic effect. |
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| ISSN: | 1561-4107 2251-7170 |