Genetic diversity of legume yellow mosaic begomoviruses in Indonesia and Vietnam

Genetic diversity of legume yellow mosaic begomoviruses in Indonesia and Vietnam

High incidences of yellow mosaic symptoms were observed in soybean and yard‐long bean crops in Indonesia in 2009 and in mungbean crops in Vietnam in 2011. All five soybean and 20 yard‐long bean samples from Java, Indonesia, and 15 mungbean samples from Vietnam with symptoms tested positive for begom...

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Bibliographic Details
Published in:Annals of applied biology Vol. 163; no. 3; pp. 367 - 377
Main Authors: Tsai, W.S, Shih, S.L, Rauf, A, Safitri, R, Hidayati, N, Huyen, B.T.T, Kenyon, L
Format: Journal Article
Language:English
Published: Oxford, UK Blackwell Publishing Ltd 01-11-2013
Blackwell
Wiley Subscription Services, Inc
Subjects:
Agronomy. Soil science and plant productions
Beans
Begomovirus
Biological and medical sciences
crops
Fundamental and applied biological sciences. Psychology
Geminiviridae
Genetic diversity
genetic variation
Glycine max
Microbiology
mung beans
Mungbean yellow mosaic India virus
Mungbean yellow mosaic virus
nucleotide sequences
phylogenetic analysis
phylogeny
Phytopathology. Animal pests. Plant and forest protection
Plant viruses and viroids
polymerase chain reaction
sequence analysis
soybeans
Vigna radiata
Vigna unguiculata subsp. sesquipedalis
South east Asia
Vietnam
Asia
Indonesia
Plant pathogen
Tropical zone
Vigna unguiculata
Phylogeny
Biodiversity
Begomovirus
phylogenetic analysis
Dicotyledones
Angiospermae
Geminiviridae
C3-Type
DNA virus
Vigna unguiculata subsp. sesquipedalis
Plant production
Vegetals
Vigna radiata
Genetic diversity
Glycine max
Virus
Polymerase chain reaction
Grain legume
Applied biology
Leguminosae
Vegetable crop
Analysis
Spermatophyta
Molecular biology
Oil plant (vegetal)
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Summary:High incidences of yellow mosaic symptoms were observed in soybean and yard‐long bean crops in Indonesia in 2009 and in mungbean crops in Vietnam in 2011. All five soybean and 20 yard‐long bean samples from Java, Indonesia, and 15 mungbean samples from Vietnam with symptoms tested positive for begomovirus infection by polymerase chain reaction (PCR) with primer pair PAL1v1978B/PAR1c715H. On the basis of collection location and the nucleotide sequence comparisons of the 1.5 kb begomoviral DNA‐A components amplified, a subset of samples comprising two soybean and six yard‐long bean isolates from Indonesia and five mungbean isolates from Vietnam were taken forward for more detailed examination. Sequence comparison and phylogenetic analysis of the full‐length sequences of all Indonesian and Vietnam isolates alongside other legume‐infecting begomoviruses revealed that all the isolates from Indonesia were Mungbean yellow mosaic India virus (MYMIV) strain‐A, and all from Vietnam were Mungbean yellow mosaic virus (MYMV) strain‐B. To the best of our knowledge, this is the first identification of MYMIV and MYMV associated with yellow mosaic of legumes in Indonesia and Vietnam, respectively. The epidemiological implications and potential consequences of the emergence of legume‐infecting begomoviruses on legume production in these areas of Southeast Asia are discussed.
Bibliography:http://dx.doi.org/10.1111/aab.12063
istex:2495B9083EFEB69FA8934B8B9442A95F1AC13DFF
ark:/67375/WNG-0DKLKB1V-C
Table S1. Details, including GenBank accession numbers of DNA-As and -Bs, of legume-infecting begomoviruses used in the phylogenetic studyTable S2. Sequences of PCR primers used to amplify DNA-A and DNA-B of MYMV and MYMIV from Vietnam and Indonesia
ArticleID:AAB12063
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0003-4746
1744-7348
DOI:10.1111/aab.12063