Search Results - "Hutchinson, Nancy I"

Active-Site Structure Analysis of Recombinant Human Inducible Nitric Oxide Synthase Using Imidazole by Chabin, Renee M, McCauley, Ermenegilda, Calaycay, Jimmy R, Kelly, Theresa M, MacNaul, Karen L, Wolfe, Gloria C, Hutchinson, Nancy I, Madhusudanaraju, Sayyaparaju, Schmidt, John A, Kozarich, John W, Wong, Kenny K

“…Nitric oxide synthase catalyzes the pyridine nucleotide-dependent oxidation of l-arginine to nitric oxide and l-citrulline. It is a specialized cytochrome P450…”
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Human fibroblast stromelysin catalytic domain: expression, purification, and characterization of a C-terminally truncated form by Marcy, Alice I, Eiberger, Laura L, Harrison, Richard, Chan, H. Karen, Hutchinson, Nancy I, Hagmann, William K, Cameron, Patricia M, Boulton, David A, Hermes, Jeffrey D

“…Stromelysin-1 is a member of a tissue metalloproteinase family whose members are all capable of degrading extracellular matrix components. A truncated form of…”
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Expression and Immunoaffinity Purification of Human Inducible Nitric-oxide Synthase by Calaycay, Jimmy R., Kelly, Theresa M., MacNaul, Karen L., McCauley, Ermenegilda D., Qi, Hongbo, Grant, Stephan K., Griffin, Patrick R., Klatt, Tracey, Raju, S.M., Nussler, Andreas K., Shah, Shrenik, Weidner, Jeffrey R., Williams, Hollis R., Wolfe, Gloria C., Geller, David A., Billiar, Timothy R., MacCoss, Malcolm, Mumford, Richard A., Tocci, Michael J., Schmidt, John A., Wong, Kenny K., Hutchinson, Nancy I.

“…Recombinant human inducible nitric-oxide synthase (rH-iNOS) was expressed in the baculovirus system and purified by a novel immunoaffinity column. rH-iNOS and…”
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Aggrecan degradation in human cartilage. Evidence for both matrix metalloproteinase and aggrecanase activity in normal, osteoarthritic, and rheumatoid joints by Lark, M W, Bayne, E K, Flanagan, J, Harper, C F, Hoerrner, L A, Hutchinson, N I, Singer, I I, Donatelli, S A, Weidner, J R, Williams, H R, Mumford, R A, Lohmander, L S

“…To examine the activity of matrix metalloproteinases (MMPs) and aggrecanase in control and diseased human articular cartilage, metabolic fragments of aggrecan…”
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Differential expression of iNOS and cNOS mRNA in human vascular smooth muscle cells and endothelial cells under normal and inflammatory conditions by MacNaul, K L, Hutchinson, N I

“…To examine the potential contribution of endothelial cell cNOS (ec-cNOS) and inducible NOS (iNOS) in controlling vascular tone under normal versus inflammatory…”
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Molecular cloning, structure, and chromosomal localization of the human inducible nitric oxide synthase gene by CHARTRAIN, N. A, GELLER, D. A, KOTY, P. P, SITRIN, N. F, NUSSLER, A. K, HOFFMAN, E. P, BILLIAR, T. R, HUTCHINSON, N. I, MUDGETT, J. S

“…Nitric oxide, a multifunctional effector molecule synthesized by nitric oxide synthase (NOS) from L-arginine, conveys signals for vasorelaxation,…”
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Discoordinate expression of stromelysin, collagenase, and tissue inhibitor of metalloproteinases-1 in rheumatoid human synovial fibroblasts. Synergistic effects of interleukin-1 and tumor necrosis factor-alpha on stromelysin expression by MacNaul, K L, Chartrain, N, Lark, M, Tocci, M J, Hutchinson, N I

“…Primary and passaged human synovial fibroblasts isolated from rheumatoid pannus were treated with recombinant interleukin-1 (IL-1) alpha or beta, tumor…”
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The potential role of fibroblasts in periprosthetic osteolysis: Fibroblast response to titanium particles by Yao, Jianling, Glant, Tibor T., Lark, Michael W., Mikecz, Katalin, Jacobs, Joshua J., Hutchinson, Nancy I., Hoerrner, Lori A., Kuettner, Klaus E., Galante, Jorge O.

“…Periprosthetic osteolysis with or without aseptic loosening is a major clinical problem in total hip arthroplasty. While the macrophage response to prosthetic…”
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Analysis of IL-1 and TNF-alpha gene expression in human rheumatoid synoviocytes and normal monocytes by in situ hybridization by MacNaul, KL, Hutchinson, NI, Parsons, JN, Bayne, EK, Tocci, MJ

“…Human IL-1 alpha, IL-1 beta, and TNF-alpha mRNA expression was examined in peripheral blood monocytes (PBM) from normal individuals and in primary synoviocytes…”
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Expression of 92-kD type IV collagenase/gelatinase (gelatinase B) in osteoarthritic cartilage and its induction in normal human articular cartilage by interleukin 1 by MOHTAI, M, SMITH, R. L, SCHURMAN, D. J, TSUJI, Y, TORTI, F. M, HUTCHINSON, N. I, STETLER-STEVENSON, W. G, GOLDBERG, G. I

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Expression and immunoaffinity purification of human inducible nitric-oxide synthase. Inhibition studies with 2-amino-5,6-dihydro-4H-1,3-thiazine by Calaycay, J R, Kelly, T M, MacNaul, K L, McCauley, E D, Qi, H, Grant, S K, Griffin, P R, Klatt, T, Raju, S M, Nussler, A K, Shah, S, Weidner, J R, Williams, H R, Wolfe, G C, Geller, D A, Billiar, T R, MacCoss, M, Mumford, R A, Tocci, M J, Schmidt, J A, Wong, K K, Hutchinson, N I

“…Recombinant human inducible nitric-oxide synthase (rH-iNOS) was expressed in the baculovirus system and purified by a novel immunoaffinity column. rH-iNOS and…”
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Organization and expression of the major genes from the long inverted repeat of the human cytomegalovirus genome by Hutchinson, N I, Sondermeyer, R T, Tocci, M J

“…The long inverted repeat (TRL:IRL) of the human cytomegalovirus (CMV) genome is a major transcription unit in productively infected human fibroblasts. Cloned…”
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Characterization of a major early gene from the human cytomegalovirus long inverted repeat; predicted amino acid sequence of a 30-kDa protein encoded by the 1.2-kb mRNA by Hutchinson, N I, Tocci, M J

“…The human cytomegalovirus (CMV) long inverted repeat (TRL:IRL) encodes three major early mRNAs. One of these RNAs is a 1.2-kb transcript that maps between…”
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Transformation by purified early genes of simian virus 40 by Chang, L S, Pater, M M, Hutchinson, N I, di Mayorca, G

“…A rapid soft-agar assay using baby hamster kidney (BHK21 cl.13) cells has been developed to establish the functional roles for the large T and small t antigens…”
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Differential in vivo expression of collagenase messenger RNA in synovium and cartilage. Quantitative comparison with stromelysin messenger RNA levels in human rheumatoid arthritis and osteoarthritis patients and in two animal models of acute inflammatory arthritis by Wolfe, G C, MacNaul, K L, Buechel, F F, McDonnell, J, Hoerrner, L A, Lark, M W, Moore, V L, Hutchinson, N I

“…To compare quantitatively the in vivo expression of collagenase messenger RNA (mRNA) and stromelysin mRNA in the joint tissues of human osteoarthritis (OA) and…”
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In vivo expression of stromelysin in synovium and cartilage of rabbits injected intraarticularly with interleukin-1 beta by Hutchinson, N I, Lark, M W, MacNaul, K L, Harper, C, Hoerrner, L A, McDonnell, J, Donatelli, S, Moore, V, Bayne, E K

“…To examine the in vivo expression of the matrix metalloproteinase stromelysin in the synovium and articular cartilage of rabbits injected intraarticularly with…”
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Use of an antibody against the matrix metalloproteinase-generated aggrecan neoepitope FVDIPEN-COOH to assess the effects of stromelysin in a rabbit model of cartilage degradation by Bayne, E K, MacNaul, K L, Donatelli, S A, Christen, A, Griffin, P R, Hoerrner, L A, Calaycay, J R, Ayala, J M, Chapman, K, Hagmann, W

“…To define the stromelysin cleavage site in the interglobular domain of rabbit aggrecan, and to determine whether the stromelysin-generated neoepitope can be…”
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Production, purification and characterization of canine prostromelysin by Bayne, E K, Hutchinson, N I, Walakovits, L A, Donatelli, S, MacNaul, K L, Harper, C F, Cameron, P, Moore, V L, Lark, M W

“…One of the best studied animal models of osteoarthritis is a dog model in which the anterior cruciate ligament of the hind limb stifle joint is transected…”
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