Lipidomic analysis reveals the efficiency of Eclipta prostrata on diet-induced nonalcoholic fatty liver disease in rats

•Lipidomics is a potential recent approach for early detection and prognosis of NAFLD.•Cholest-5-en-3-ol (3β) – acetate; glycerol; linoleic acid and arachidonic acid are novel biomarkers for prognosis of NAFLD.•E. prostrata showed a significant amelioration of the lipidomic markers in both protectiv...

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Bibliographic Details
Published in:	Journal of pharmaceutical and biomedical analysis Vol. 165; pp. 224 - 232
Main Authors:	Hussein, Noha Said, S. Helmy, Azza, Sherif, Naglaa M., Ghanem, Hassan Z., A. Ibrahim, Nabaweya, El Gendy, Abdel Nasser G., Abdel-Hamid, Abdel-Hamid Z.
Format:	Journal Article
Language:	English
Published:	England Elsevier B.V 20-02-2019
Subjects:	Animals Biomarkers - metabolism Chromatography, High Pressure Liquid - methods Diet, High-Fat - adverse effects Dose-Response Relationship, Drug Eclipta - chemistry Eclipta prostrata Gas Chromatography-Mass Spectrometry - methods GC–MS Lipidomics biomarkers Lipids - blood Male Multivariate Analysis NAFLD Non-alcoholic Fatty Liver Disease - drug therapy Non-alcoholic Fatty Liver Disease - physiopathology Plant Extracts - administration & dosage Plant Extracts - pharmacology Rats Rats, Wistar FFA NAFLD SR-BI LDL-C ALT LCAT Lipidomics biomarkers HMGCR Eclipta prostrata HDL-C LDLR HPLC SS ACC GC–MS AST PLS-DA HMG CoA DAG PPARα NASH SREBP-1c PCA TC MS-grade H2O AMPK TAG GPAT
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Summary:	•Lipidomics is a potential recent approach for early detection and prognosis of NAFLD.•Cholest-5-en-3-ol (3β) – acetate; glycerol; linoleic acid and arachidonic acid are novel biomarkers for prognosis of NAFLD.•E. prostrata showed a significant amelioration of the lipidomic markers in both protective and treated rats with NAFLD.•E. prostrata could be used as protective and/or therapeutic agents for NAFLD management. Non-alcoholic fatty liver disease is a leading cause of chronic liver disease in western countries. The current study aimed to detect and evaluate lipidomic biomarkers for early detection of NAFLD as well as the potential efficiency of methanolic extract of Eclipta prostrata (E. prostrata) on disease management. In this study, Phytochemical screening of E. prostrata methanolic extract was performed using HPLC. NAFLD was induced in albino rats using a high-fat diet together with cholesterol and cholic acid. Comprehensive lipidomic analyses on sera from rats bearing NAFLD as well as normal healthy animals were carried out based on GCMS and multivariate data analysis. The results showed that high doses (300&200 mg/kg.BW) of E. prostrata extract exhibited significant improvement in liver enzymes (ALT & AST) and lipid profile [total cholesterol (TC), triacylglycerides (TAGs), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein-cholesterol (LDL-C)] in rats bearing NAFLD. Glycerol, linoleic acid, arachidonic acid and cholest-5-en-3-ol (3β) acetate were detected as lipidomic biomarkers for early detection of NAFLD in rats’ sera. Furthermore, E. prostrata extract showed a significant amelioration in the levels of these metabolic biomarkers in both protective and treated groups. These finding devoutly recommend using of lipidomic biomarkers for early detection of NAFLD and E. prostrata could be used as a protective agent as well as ameliorate this disease through its probable action on the fore-mentioned metabolites.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0731-7085 1873-264X
DOI:	10.1016/j.jpba.2018.11.060