In vitro propagation of a grape rootstock, deGrasset ( Vitis champinii Planch.): Effects of medium compositions and plant growth regulators

The aim of the present study was to develop a protocol for in vitro propagation of a grape rootstock, deGrasset, characterized by high tolerance to drought and salinity. Four medium compositions, MS, MS with 1/2 nitrates (MS-1), B5 and WPM, were tested for shoot growth from nodal explants and MS-1 m...

Full description

Saved in:

Bibliographic Details
Published in:	Scientia horticulturae Vol. 126; no. 1; pp. 13 - 19
Main Authors:	Mukherjee, Papiya, Husain, Nafisa, Misra, S.C., Rao, V.S.
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier B.V 01-08-2010 [Amsterdam; New York, NY]: Elsevier Science Elsevier
Subjects:	acclimation Agronomy. Soil science and plant productions benzyladenine Biological and medical sciences callus formation culture media cytokinins deGrasset Fundamental and applied biological sciences. Psychology General agronomy. Plant production grapes Hyperhydricity In vitro propagation indole acetic acid indole butyric acid Medium composition micropropagation naphthaleneacetic acid optimization organogenesis Plant growth regulators plant growth substances plantlets Rooting roots rootstocks shoots thidiazuron Vegetative propagation. Micropropagation Vegetative propagation. Sowing and planting. Harvesting Vitis champini Vitis vinifera zeatin In vitro propagation deGrasset MS Medium composition IAA IBA TDZ DMRT NAA BAP B5 Plant growth regulators WPM Hyperhydricity ZEA Rooting Horticulture Micropropagation Rootstock Vitidaceae Dicotyledones Angiospermae Plant growth substance Spermatophyta
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	The aim of the present study was to develop a protocol for in vitro propagation of a grape rootstock, deGrasset, characterized by high tolerance to drought and salinity. Four medium compositions, MS, MS with 1/2 nitrates (MS-1), B5 and WPM, were tested for shoot growth from nodal explants and MS-1 medium produced significantly higher rate of shoot proliferation. MS-1 medium supplemented with 2.0 mg/L BAP was found to be optimum for culture establishment. The first subculturing on the same medium resulted in the production of 4–6, mostly short, hyperhydrated shoots per explant. For subsequent subcultures, a reduced concentration of BAP (1.0 mg/L) was used to prevent hyperhydricity, and that resulted in distinct individual shoot elongation. Three plant growth regulators, BAP, ZEA and TDZ, were tested for further shoot proliferation and BAP at 1.0 mg/L added to MS-1 medium displayed the highest proliferation rate (4.75 new explants per explant inoculated, in 6 weeks). For in vitro rooting of shoots, IAA at 0.2 mg/L was found to be optimum to produce highest response (84%) and an average number of 2.03 roots per shoot whereas use of IBA or NAA resulted in rooting with high frequency of callus formation. The acclimatized plantlets were established in soil under net house conditions with 87% success.
Bibliography:	http://dx.doi.org/10.1016/j.scienta.2010.06.002
ISSN:	0304-4238 1879-1018
DOI:	10.1016/j.scienta.2010.06.002