Search Results - "Hurley, James H"

ESCRTs are everywhere by Hurley, James H

“…The ESCRT proteins are an ancient system that buds membranes and severs membrane necks from their inner face. Three “classical” functions of the ESCRTs have…”
Get full text

Mechanisms of Autophagy Initiation by Hurley, James H, Young, Lindsey N

“…Autophagy is the process of cellular self-eating by a double-membrane organelle, the autophagosome. A range of signaling processes converge on two protein…”
Get full text

Molecular mechanism of multivesicular body biogenesis by ESCRT complexes by Hurley, James H, Wollert, Thomas

“…When internalized receptors and other cargo are destined for lysosomal degradation, they are ubiquitinated and sorted by the endosomal sorting complex required…”
Get full text

ESCRT complexes and the biogenesis of multivesicular bodies by Hurley, James H

“…Multivesicular bodies (MVBs) are crucial intermediates in the trafficking of ubiquitinated receptors and other cargo destined for lysosomes. The formation of…”
Get full text

Structure and function of the ULK1 complex in autophagy by Lin, Mary G, Hurley, James H

“…The ULK1 complex initiates autophagosome formation, linking cellular nutrient status to downstream events in autophagy. Recent work suggests that the ULK1…”
Get full text

Atomistic autophagy: the structures of cellular self-digestion by Hurley, James H, Schulman, Brenda A

“…Autophagy is directed by numerous distinct autophagy-related (Atg) proteins. These transmit starvation-induced signals to lipids and regulatory proteins and…”
Get full text

Membrane budding and scission by the ESCRT machinery: it's all in the neck by Hurley, James H, Hanson, Phyllis I

“…Key Points Endosomal sorting complexes required for transport (ESCRTs) are required for the lysosomal degradation of plasma membrane proteins, budding of most…”
Get full text

Structural mechanism for HIV-1 TAR loop recognition by Tat and the super elongation complex by Schulze-Gahmen, Ursula, Hurley, James H.

“…Promoter-proximal pausing by RNA polymerase II (Pol II) is a key regulatory step in human immunodeficiency virus-1 (HIV-1) transcription and thus in the…”
Get full text

Two-Site Recognition of Phosphatidylinositol 3-Phosphate by PROPPINs in Autophagy by Baskaran, Sulochanadevi, Ragusa, Michael J., Boura, Evzen, Hurley, James H.

“…Macroautophagy is essential to cell survival during starvation and proceeds by the growth of a double-membraned phagophore, which engulfs cytosol and other…”
Get full text

The ESCRTs - converging on mechanism by Remec Pavlin, Mark, Hurley, James H

“…The endosomal sorting complexes required for transport (ESCRTs) I, -II and -III, and their associated factors are a collection of ∼20 proteins in yeast and ∼30…”
Get full text

Ubiquitin-binding domains by Hurley, James H, Lee, Sangho, Prag, Gali

“…The covalent modification of proteins by ubiquitination is a major regulatory mechanism of protein degradation and quality control, endocytosis, vesicular…”
Get full text

Structure of SARS-CoV-2 ORF8, a rapidly evolving immune evasion protein by Flower, Thomas G., Buffalo, Cosmo Z., Hooy, Richard M., Allaire, Marc, Ren, Xuefeng, Hurley, James H.

“…The molecular basis for the severity and rapid spread of the COVID-19 disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is largely…”
Get full text

A PI3K-WIPI2 positive feedback loop allosterically activates LC3 lipidation in autophagy by Fracchiolla, Dorotea, Chang, Chunmei, Hurley, James H, Martens, Sascha

“…Autophagy degrades cytoplasmic cargo by its delivery to lysosomes within double membrane autophagosomes. Synthesis of the phosphoinositide PI(3)P by the…”
Get full text

Architecture of the Atg17 Complex as a Scaffold for Autophagosome Biogenesis by Ragusa, Michael J., Stanley, Robin E., Hurley, James H.

“…Macroautophagy is a bulk clearance mechanism in which the double-membraned phagophore grows and engulfs cytosolic material. In yeast, the phagophore nucleates…”
Get full text

Crystallographic molecular replacement using an in silico‐generated search model of SARS‐CoV‐2 ORF8 by Flower, Thomas G., Hurley, James H.

“…The majority of crystal structures are determined by the method of molecular replacement (MR). The range of application of MR is limited mainly by the need for…”
Get full text

Structural Basis for Recruitment and Activation of the AP-1 Clathrin Adaptor Complex by Arf1 by Ren, Xuefeng, Farías, Ginny G., Canagarajah, Bertram J., Bonifacino, Juan S., Hurley, James H.

“…AP-1 is a clathrin adaptor complex that sorts cargo between the trans-Golgi network and endosomes. AP-1 recruitment to these compartments requires Arf1-GTP…”
Get full text

Vps34 Kinase Domain Dynamics Regulate the Autophagic PI 3-Kinase Complex by Stjepanovic, Goran, Baskaran, Sulochanadevi, Lin, Mary G., Hurley, James H.

“…The class III phosphatidylinositol 3-kinase complex I (PI3KC3-C1) is required for the initiation of essentially all macroautophagic processes. PI3KC3-C1…”
Get full text

Longin domain GAP complexes in nutrient signalling, membrane traffic and neurodegeneration by Jansen, Rachel M., Hurley, James H.

“…Small GTPases act as molecular switches and control numerous cellular processes by their binding and hydrolysis of guanosine triphosphate (GTP). The activity…”
Get full text

Structure of the lysosomal mTORC1–TFEB–Rag–Ragulator megacomplex by Cui, Zhicheng, Napolitano, Gennaro, de Araujo, Mariana E. G., Esposito, Alessandra, Monfregola, Jlenia, Huber, Lukas A., Ballabio, Andrea, Hurley, James H.

“…The transcription factor TFEB is a master regulator of lysosomal biogenesis and autophagy 1 . The phosphorylation of TFEB by the mechanistic target of…”
Get full text

VHS domains of ESCRT-0 cooperate in high-avidity binding to polyubiquitinated cargo by Ren, Xuefeng, Hurley, James H

“…VHS (Vps27, Hrs, and STAM) domains occur in ESCRT‐0 subunits Hrs and STAM, GGA adapters, and other trafficking proteins. The structure of the STAM VHS…”
Get full text