Report of the cyanotoxins cylindrospermopsin and deoxy-cylindrospermopsin from Raphidiopsis mediterranea Skuja (Cyanobacteria/Nostocales)

▸ We isolated a strain of the bloom forming cyanobacterium Raphidiopsis mediterranea Skuja. ▸ Evidence is provided which shows that it produces the cyanotoxins cylindrospermopsin and deoxy-cylindrospermopsin. ▸ This is the first evidence of the production of cyanotoxins by this species. A filamentou...

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Bibliographic Details
Published in:	Harmful algae Vol. 10; no. 4; pp. 402 - 410
Main Authors:	McGregor, Glenn B., Sendall, Barbara C., Hunt, Lindsay T., Eaglesham, Geoffrey K.
Format:	Journal Article
Language:	English
Published:	Kidlington Elsevier B.V 01-05-2011 Elsevier
Subjects:	Algae Animal and plant ecology Animal, plant and microbial ecology Autoecology Biological and medical sciences biosynthesis Cyanobacteria Cyanobacterium Cyanotoxin cylindrospermopsin Cylindrospermopsin (CYN) Deoxy-cylindrospermopsin (deoxy-CYN) Fundamental and applied biological sciences. Psychology genes high performance liquid chromatography Nostocales phylogeny plankton Plant cytology, morphology, systematics, chorology and evolution Plants and fungi polymerase chain reaction Raphidiopsis Raphidiopsis mediterranea ribosomal RNA Thallophyta Queensland Australia, Queensland Deoxy-cylindrospermopsin (deoxy-CYN) Cylindrospermopsin (CYN) Raphidiopsis mediterranea Cyanobacteria Cyanotoxin Aquatic environment Algae Bacteria Harmful alga
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Summary:	▸ We isolated a strain of the bloom forming cyanobacterium Raphidiopsis mediterranea Skuja. ▸ Evidence is provided which shows that it produces the cyanotoxins cylindrospermopsin and deoxy-cylindrospermopsin. ▸ This is the first evidence of the production of cyanotoxins by this species. A filamentous cyanobacterium isolated from the plankton of a eutrophic reservoir in Queensland (Australia) was, based on morphological features, putatively identified as Raphidiopsis mediterranea Skuja. Strain FSS1-150/1 was screened by HPLC MS/MS for a number of common cyanotoxins and was shown to produce both cylindrospermopsin (CYN) and deoxy-cylindrospermopsin (deoxy-CYN) at concentrations of 917 and 1065μgg−1 dry weight respectively. Evidence for CYN synthesis by strain FSS1-150/1 was further supported by PCR amplification of a fragment of the CyrC gene involved in CYN biosynthesis. Subsequent phylogenetic analyses using a partial sequence of the 16S rRNA gene confirmed its identification and showed that strain FSS1-150/1 was closely related to strains of R. mediterranea and R. curvata. These results provide the first evidence of the production of CYN and deoxy-CYN by R. mediterranea and it is the second species of the genus Raphidiopsis known to produce these potent cyanotoxins.
Bibliography:	http://dx.doi.org/10.1016/j.hal.2011.02.002 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1568-9883 1878-1470
DOI:	10.1016/j.hal.2011.02.002