Search Results - "Hughes, Karin A"

Phenylboronic Acid−Salicylhydroxamic Acid Bioconjugates. 1. A Novel Boronic Acid Complex for Protein Immobilization by Stolowitz, Mark L, Ahlem, Clarence, Hughes, Karin A, Kaiser, Robert J, Kesicki, Edward A, Li, Guisheng, Lund, Kevin P, Torkelson, Steven M, Wiley, Jean P

“…A chemical affinity system exhibiting antibody-like properties is described. The system exploits bioconjugates with appended phenylboronic acid (PBA) moieties…”
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Use of a small molecule-based affinity system for the preparation of protein microarrays by Hughes, Karin A

“…This chapter describes a method for preparing protein microarrays, using a small-molecule, chemical affinity system…”
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Small-molecule affinity-based matrices for rapid protein purification by Hughes, Karin A, Wiley, Jean P

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Phenylboronic Acid−Salicylhydroxamic Acid Bioconjugates. 2. Polyvalent Immobilization of Protein Ligands for Affinity Chromatography by Wiley, Jean P, Hughes, Karin A, Kaiser, Robert J, Kesicki, Edward A, Lund, Kevin P, Stolowitz, Mark L

“…Phenylboronic acid bioconjugates prepared from alkaline phosphatase by reaction with either 2,5-dioxopyrrolidinyl…”
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Salicylhydroxamic acid functionalized affinity membranes for specific immobilization of proteins and oligonucleotides by Springer, Amy L, Gall, Anna S, Hughes, Karin A, Kaiser, Robert J, Li, Guisheng, Lund, Kevin P

“…Immobilization of proteins and other biological macromolecules on solid supports is a method suitable for purification or screening applications in life…”
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A rapid method for manual or automated purification of fluorescently labeled nucleic acids for sequencing, genotyping, and microarrays by Springer, Amy L, Booth, Lisa R, Braid, Michael D, Houde, Christiane M, Hughes, Karin A, Kaiser, Robert J, Pedrak, Casandra, Spicer, Douglas A, Stolyar, Sergey

“…Fluorescent dyes provide specific, sensitive, and multiplexed detection of nucleic acids. To maximize sensitivity, fluorescently labeled reaction products…”
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A new condensation synthesis of allenes and dienes by Reynolds, Kelly A, Dopico, Pablo G, Sundermann, Michael J, Hughes, Karin A, Finn, M. G

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Stability of pentaammineosmium(II) in aqueous solution by Call, Jason T, Hughes, Karin A, Harman, W. Dean, Finn, M. G

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Binding of the sigma70 protein to the core subunits of Escherichia coli RNA polymerase, studied by iron-EDTA protein footprinting by Greiner, Douglas P, Hughes, Karin A, Gunasekera, Angelo H, Meares, Claude F

“…Greiner et al use a nonspecific protein cleaving reagent to map the interactions between subunits of the multisubunit enzyme RNA polymerase (Escherichia coli)…”
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Binding of the σ70Protein to the Core Subunits of Escherichia coli RNA Polymerase, Studied by Iron-EDTA Protein Footprinting by Greiner, Douglas P., Hughes, Karin A., Gunasekera, Angelo H., Meares, Claude F.

“…We have used a nonspecific protein cleaving reagent to map the interactions between subunits of the multisubunit enzyme RNA polymerase (Escherichia coli). We…”
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Radiolytic Protein Surface Mapping by Greiner, Douglas P., Hughes, Karin A., Meares, Claude F.

“…We report that high energy beta particles may function as a means for mapping the surface of a protein. Comparable to Fe-EDTA in the presence of ascorbate and…”
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A Fully Automated Process Using a Magnetic Particle Based Kit for Removal of Dye Terminators from Sequencing Reactions by Spicer, Douglas A., Booth, Lisa R., Hughes, Karin A., Kaiser, Robert J., Springer, Amy L.

“…Prolinx,® Inc. of Bothell, WA has developed the RapXtract™ 384 Dye Terminator Removal Kit for full automation of DNA sequencing reaction purification. The…”
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A 384-Well, Fully-Automatable, Superparamagnetic-Particle Based Dye Terminator Removal Kit by Spicer, Douglas A., Astle, Thomas, Booth, Lisa R., House, Russell, Hughes, Karin A., Kaiser, Robert J., Springer, Amy L.

“…We have developed a method for the automated purification of DNA sequencing reactions using the RapXtract(tm) 384 Dye Terminator Removal Kit and the Quadra…”
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