Transcripts of MYB-like genes respond to phosphorous and nitrogen deprivation in Arabidopsis

In Arabidopsis thaliana (L.) Heynh., AtPhr2 and AtNsr1 encode proteins with MYB-like and α-helical domains. They resemble CrPsr1, a nuclear-localized MYB protein that is critical for acclimation to phosphorous (P) starvation in the alga Chlamydomonas reinhardtii. Reverse transcription-polymerase cha...

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Bibliographic Details
Published in:	Planta Vol. 219; no. 6; pp. 1003 - 1009
Main Authors:	Todd, C.D, Zeng, P, Huete, A.M.R, Hoyos, M.E, Polacco, J.C
Format:	Journal Article
Language:	English
Published:	Berlin Springer-Verlag 01-10-2004 Springer Springer Nature B.V
Subjects:	Acclimatization acid phosphatase Acid Phosphatase - biosynthesis Amino Acid Sequence amino acid sequences Arabidopsis - genetics Arabidopsis - metabolism Arabidopsis Proteins - genetics Arabidopsis thaliana Biological and medical sciences Chromosome Mapping Complementary DNA Fundamental and applied biological sciences. Psychology gene expression regulation Gene Expression Regulation, Plant - physiology Genes Genes. Genome Glycine max insertional mutagenesis messenger RNA Molecular and cellular biology Molecular genetics Molecular Sequence Data Mutation MYB-like protein nitrate transporter Nitrates Nitrogen Nitrogen - metabolism nutrient solutions Nutrients Nutritional deficiencies Phosphatases Phosphates phosphorus Phosphorus - metabolism plant nutrition plant proteins Plant roots Plants RNA RNA, Messenger - metabolism RNA, Plant - metabolism Sequence Alignment soybeans Time Factors transfer DNA transgenic plants Transcription Enzyme Arabidopsis Algae Phosphoric monoester hydrolases Nitrogen deprivation Esterases Chlorophyceae Phosphatase Arabidopsis thaliana MYB factor Nitrate Chlorophyta Gene Cruciferae Dicotyledones Chlamydomonas reinhardtii Angiospermae Nitrate transporter Hydrolases Spermatophyta Mutation Acid phosphatase Thallophyta
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Summary:	In Arabidopsis thaliana (L.) Heynh., AtPhr2 and AtNsr1 encode proteins with MYB-like and α-helical domains. They resemble CrPsr1, a nuclear-localized MYB protein that is critical for acclimation to phosphorous (P) starvation in the alga Chlamydomonas reinhardtii. Reverse transcription-polymerase chain reaction analysis of the first unique exons indicated that AtPhr2 mRNA increased as early as 6 h after P deprivation (-P), whereas nitrogen deprivation (-N) had no effect. The AtNsr1 mRNA level increased exclusively under -N, an increase first noted by 2 days in -N. In spite of P- and N-specific effects on expression of AtPhr2 and AtNsr1 there appeared to be P-N cross-talk at the whole-plant level. Total non-secreted acid phosphatase activity increased under both -P and -N within 2 days of deprivation. Further, the pho2-1/pho2-1 mutant, reported to be a phosphate accumulator, showed no increase in AtPhr2 mRNA in response to -P and a 70% reduction in the response of AtNsr1 mRNA to -N. Consistent with this pattern, there was no increase in acid phosphatase activity in pho2-1/pho2-1 plants deprived of P or N. However, when deprived of P, pho2-1/pho2-1 plants accumulated much higher levels of nitrate. T-DNA disruption of AtNsr1 resulted in altered expression of at least one nitrate transporter (AtNRT2.5). Further evidence of cross-talk between N and P responses was altered expression of N-responsive genes in pho2-1/pho2-1.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0032-0935 1432-2048
DOI:	10.1007/s00425-004-1305-7