Ultra-fast, high throughput and inexpensive detection of SARS-CoV-2 seroconversion using Ni2+ magnetic beads

Ultra-fast, high throughput and inexpensive detection of SARS-CoV-2 seroconversion using Ni2+ magnetic beads

To monitor the levels of protecting antibodies raised in the population in response to infection and/or to immunization with SARS-CoV-2, we need a technique that allows high throughput and low-cost quantitative analysis of human IgG antibodies reactive against viral antigens. Here we describe an ult...

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Bibliographic Details
Published in:Analytical biochemistry Vol. 631; p. 114360
Main Authors: Conzentino, Marcelo S., Santos, Tatielle P.C., Selim, Khaled A., Wagner, Berenike, Alford, Janette T., Deobald, Nelli, Paula, Nigela M., Rego, Fabiane G.M., Zanette, Dalila L., Aoki, Mateus N., Nardin, Jeanine M., Huergo, Maria C.C., Reis, Rodrigo A., Huergo, Luciano F.
Format: Journal Article
Language:English
Published: Elsevier Inc 15-10-2021
Subjects:
COVID-19
High throughput
Magnetic beads
Magnetic ELISA
SARS-CoV-2
COVID-19
Magnetic ELISA
High throughput
Magnetic beads
SARS-CoV-2
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Summary:To monitor the levels of protecting antibodies raised in the population in response to infection and/or to immunization with SARS-CoV-2, we need a technique that allows high throughput and low-cost quantitative analysis of human IgG antibodies reactive against viral antigens. Here we describe an ultra-fast, high throughput and inexpensive assay to detect SARS-CoV-2 seroconversion in humans. The assay is based on Ni2+ magnetic particles coated with His tagged SARS-CoV-2 antigens. A simple and inexpensive 96 well plate magnetic extraction/homogenization process is described which allows the simultaneous analysis of 96 samples and delivers results in 7 min with high accuracy. [Display omitted] •A system enabling high throughput magnetic bead transfer and homogenization is described.•Such system allowed high throughput analysis of SARS-CoV-2 seroconversion in humans.•Up to 96 samples can be analyzed for IgG in less than 7 min.•The system can be used with any His tagged protein as antigen.
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ISSN:0003-2697
1096-0309
DOI:10.1016/j.ab.2021.114360