Search Results - "Hudson, Elton P."

Diversion of the long-chain acyl-ACP pool in Synechocystis to fatty alcohols through CRISPRi repression of the essential phosphate acyltransferase PlsX by Kaczmarzyk, Danuta, Cengic, Ivana, Yao, Lun, Hudson, Elton P.

“…Fatty alcohol production in Synechocystis sp. PCC 6803 was achieved through heterologous expression of the fatty acyl-CoA/ACP reductase Maqu2220 from the…”
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Pooled CRISPRi screening of the cyanobacterium Synechocystis sp PCC 6803 for enhanced industrial phenotypes by Yao, Lun, Shabestary, Kiyan, Björk, Sara M., Asplund-Samuelsson, Johannes, Joensson, Haakan N., Jahn, Michael, Hudson, Elton P.

“…Cyanobacteria are model organisms for photosynthesis and are attractive for biotechnology applications. To aid investigation of genotype-phenotype…”
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Wide range of metabolic adaptations to the acquisition of the Calvin cycle revealed by comparison of microbial genomes by Asplund-Samuelsson, Johannes, Hudson, Elton P

“…Knowledge of the genetic basis for autotrophic metabolism is valuable since it relates to both the emergence of life and to the metabolic engineering challenge…”
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Growth of Cyanobacteria Is Constrained by the Abundance of Light and Carbon Assimilation Proteins by Jahn, Michael, Vialas, Vital, Karlsen, Jan, Maddalo, Gianluca, Edfors, Fredrik, Forsström, Björn, Uhlén, Mathias, Käll, Lukas, Hudson, Elton P.

“…Cyanobacteria must balance separate demands for energy generation, carbon assimilation, and biomass synthesis. We used shotgun proteomics to investigate…”
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Computational metabolic engineering strategies for growth-coupled biofuel production by Synechocystis by Shabestary, Kiyan, Hudson, Elton P.

“…Chemical and fuel production by photosynthetic cyanobacteria is a promising technology but to date has not reached competitive rates and titers. Genome-scale…”
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Overexpression of sigma factor SigB improves temperature and butanol tolerance of Synechocystis sp. PCC6803 by Kaczmarzyk, Danuta, Anfelt, Josefine, Särnegrim, Amanda, Hudson, Elton P.

“…•We overexpress or delete stress-related regulatory proteins in a model cyanobacterium.•Overexpression of the RNA Polymerase sigma factor SigB increased both…”
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Manipulation of topoisomerase expression inhibits cell division but not growth and reveals a distinctive promoter structure in Synechocystis by Behle, Anna, Dietsch, Maximilian, Goldschmidt, Louis, Murugathas, Wandana, Berwanger, Lutz C, Burmester, Jonas, Yao, Lun, Brandt, David, Busche, Tobias, Kalinowski, Jörn, Hudson, Elton P, Ebenhöh, Oliver, Axmann, Ilka M, Machné, Rainer

“…In cyanobacteria DNA supercoiling varies over the diurnal cycle and is integrated with temporal programs of transcription and replication. We manipulated DNA…”
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Genetic and nutrient modulation of acetyl-CoA levels in Synechocystis for n-butanol production by Anfelt, Josefine, Kaczmarzyk, Danuta, Shabestary, Kiyan, Renberg, Björn, Rockberg, Johan, Nielsen, Jens, Uhlén, Mathias, Hudson, Elton P

“…There is a strong interest in using photosynthetic cyanobacteria as production hosts for biofuels and chemicals. Recent work has shown the benefit of pathway…”
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Using Transcriptomics To Improve Butanol Tolerance of Synechocystis sp. Strain PCC 6803 by Anfelt, Josefine, Hallström, Björn, Nielsen, Jens, Uhlén, Mathias, Hudson, Elton P

“…Classifications Services AEM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit…”
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Slow Protein Turnover Explains Limited Protein-Level Response to Diurnal Transcriptional Oscillations in Cyanobacteria by Karlsen, Jan, Asplund-Samuelsson, Johannes, Jahn, Michael, Vitay, Dóra, Hudson, Elton P.

“…Metabolically engineered cyanobacteria have the potential to mitigate anthropogenic CO 2 emissions by converting CO 2 into renewable fuels and chemicals. Yet,…”
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The Calvin Benson cycle in bacteria: New insights from systems biology by Hudson, Elton P.

“…The Calvin Benson cycle in phototrophic and chemolithoautotrophic bacteria has ecological and biotechnological importance, which has motivated study of its…”
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CRISPRi as a Tool to Repress Multiple Copies of Extracellular Polymeric Substances (EPS)-Related Genes in the Cyanobacterium Synechocystis sp. PCC 6803 by Santos, Marina, Pacheco, Catarina C., Yao, Lun, Hudson, Elton P., Tamagnini, Paula

“…The use of the versatile cyanobacterial extracellular polymeric substances (EPS) for biotechnological/biomedical applications implies an extensive knowledge of…”
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Metabolite interactions in the bacterial Calvin cycle and implications for flux regulation by Sporre, Emil, Karlsen, Jan, Schriever, Karen, Asplund-Samuelsson, Johannes, Janasch, Markus, Strandberg, Linnéa, Karlsson, Anna, Kotol, David, Zeckey, Luise, Piazza, Ilaria, Syrén, Per-Olof, Edfors, Fredrik, Hudson, Elton P.

“…Metabolite-level regulation of enzyme activity is important for microbes to cope with environmental shifts. Knowledge of such regulations can also guide strain…”
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Systematic overexpression study to find target enzymes enhancing production of terpenes in Synechocystis PCC 6803, using isoprene as a model compound by Englund, Elias, Shabestary, Kiyan, Hudson, Elton P., Lindberg, Pia

“…Of the two natural metabolic pathways for making terpenoids, biotechnological utilization of the mevalonate (MVA) pathway has enabled commercial production of…”
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Multiple Gene Repression in Cyanobacteria Using CRISPRi by Yao, Lun, Cengic, Ivana, Anfelt, Josefine, Hudson, Elton P

“…We describe the application of clustered regularly interspaced short palindromic repeats interference (CRISPRi) for gene repression in the model cyanobacterium…”
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Automated solid-phase subcloning based on beads brought into proximity by magnetic force by Hudson, Elton P, Nikoshkov, Andrej, Uhlen, Mathias, Rockberg, Johan

“…In the fields of proteomics, metabolic engineering and synthetic biology there is a need for high-throughput and reliable cloning methods to facilitate…”
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Kinetic modeling of the Calvin cycle identifies flux control and stable metabolomes in Synechocystis carbon fixation by Janasch, Markus, Asplund-Samuelsson, Johannes, Steuer, Ralf, Hudson, Elton P

“…An improved kinetic model of the cyanobacterial Calvin cycle employing random sampling identifies probabilistic conditions for metabolome stability and…”
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CRISPR interference screens reveal growth–robustness tradeoffs in Synechocystis sp. PCC 6803 across growth conditions by Miao, Rui, Jahn, Michael, Shabestary, Kiyan, Peltier, Gilles, Hudson, Elton P

“…Abstract Barcoded mutant libraries are a powerful tool for elucidating gene function in microbes, particularly when screened in multiple growth conditions…”
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Laboratory evolution of Synechocystis sp. PCC 6803 for phenylpropanoid production by Kukil, Kateryna, Englund, Elias, Crang, Nick, Hudson, Elton P., Lindberg, Pia

“…Cyanobacteria are promising as a biotechnological platform for production of various industrially relevant compounds, including aromatic amino acids and their…”
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Environmental impacts and limitations of third‐generation biobutanol: Life cycle assessment of n‐butanol produced by genetically engineered cyanobacteria by Nilsson, Astrid, Shabestary, Kiyan, Brandão, Miguel, Hudson, Elton P.

“…Photosynthetic cyanobacteria have attracted interest as production organisms for third‐generation biofuels, where sunlight and CO2 are used by microbes…”
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