The anti-apoptotic and anti-autophagic effects of EPO through PI3K/Akt/mTOR signaling pathway in MAC-T cells

The anti-apoptotic and anti-autophagic effects of EPO through PI3K/Akt/mTOR signaling pathway in MAC-T cells

Lipopolysaccharide (LPS) is an important inflammatory and infected factor of bacterial mastitis, which treated bovine mammary epithelial cells (MAC-T) in our previous studies, as mastitis cells model in vitro. Erythropoietin (EPO) is a well-known hematopoietic hormone with antioxidative, anti-apopto...

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Published in:Research in veterinary science Vol. 149; pp. 1 - 10
Main Authors: Liu, Jingsong, Zhang, Xueming, Yue, Shengnan, Fu, Jingcheng, Chen, Jin, Huang, Ruochao, Shang, Pengyuan, Zhong, Kai, Guo, Shuang, Jiao, Xianqin, Zha, Guangming, Han, Liqiang, Yang, Guoyu, Li, Heping, Wang, Yueying
Format: Journal Article
Language:English
Published: Oxford Elsevier Ltd 01-12-2022
Elsevier Limited
Subjects:
1-Phosphatidylinositol 3-kinase
AKT protein
Annexin V
Antibodies
Apoptosis
Autophagy
Cell growth
Cell proliferation
Epithelial cells
Epithelium
Erythropoietin
Flow cytometry
Inflammation
Kinases
Lipopolysaccharides
Lymphocytes
Lymphocytes T
MAC-T cells
Mammary gland
Mastitis
PI3K/Akt/mTOR signaling
Proteins
Reagents
Signal transduction
Signaling
TOR protein
Tumor necrosis factor-TNF
Veterinary medicine
Beijing China
United States > US
China
MAC-T cells
Inflammation
Erythropoietin
Autophagy
PI3K/Akt/mTOR signaling
Apoptosis
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Summary:Lipopolysaccharide (LPS) is an important inflammatory and infected factor of bacterial mastitis, which treated bovine mammary epithelial cells (MAC-T) in our previous studies, as mastitis cells model in vitro. Erythropoietin (EPO) is a well-known hematopoietic hormone with antioxidative, anti-apoptotic, and anti-inflammatory roles. We hypothesized that EPO might regulate the apoptosis and autophagy to attenuate the inflammation of mastitis. Western blot, RT–PCR, transmission electron microscope analysis and Annexin V-FITC/PI were used to evaluate the regulation of EPO on apoptosis and autophagy in inflammatory MAC-T cells. These results demonstrated that EPO promoted the proliferation of MAC-T cells. Meanwhile, EPO had a better anti-inflammatory effect in MAC-T cells with LPS treatment. Certainly, EPO also showed anti-apoptotic and anti-autophagic effects. Interestingly, we found that the beneficial effect of EPO on inflammatory MAC-T cells depended on the PI3K/Akt/mTOR signaling pathway, which was involved in the regulation of apoptosis and autophagy. Generally, this study provides an insight for EPO to inhibit apoptosis and autophagy of inflammatory MAC-T cells via PI3K/Akt/mTOR signaling pathway. •EPO promoted the proliferation of MAC-T cells.•EPO played a better anti-inflammatory effect in inflammatory MAC-T cells.•EPO also showed anti-apoptotic and anti-autophagic effects.•The protective effect of EPO depended on the PI3K/Akt/mTOR signaling pathway to dominate apoptosis and autophagy.
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content type line 23
ISSN:0034-5288
1532-2661
DOI:10.1016/j.rvsc.2022.06.005