Search Results - "Huang, Rachel L."

Virus-assisted directed evolution of enhanced suppressor tRNAs in mammalian cells by Jewel, Delilah, Kelemen, Rachel E., Huang, Rachel L., Zhu, Zeyu, Sundaresh, Bharathi, Cao, Xiaofu, Malley, Kaitlin, Huang, Zeyi, Pasha, Muhammad, Anthony, Jon, van Opijnen, Tim, Chatterjee, Abhishek

“…Site-specific incorporation of unnatural amino acids (Uaas) in living cells relies on engineered aminoacyl-transfer RNA synthetase–tRNA pairs borrowed from a…”
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Enhanced Directed Evolution in Mammalian Cells Yields a Hyperefficient Pyrrolysyl tRNA for Noncanonical Amino Acid Mutagenesis by Jewel, Delilah, Kelemen, Rachel E., Huang, Rachel L., Zhu, Zeyu, Sundaresh, Bharathi, Malley, Kaitlin, Pham, Quan, Loynd, Conor, Huang, Zeyi, Opijnen, Tim, Chatterjee, Abhishek

“…Heterologous tRNAs used for noncanonical amino acid (ncAA) mutagenesis in mammalian cells typically show poor activity. We recently introduced a virus‐assisted…”
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Structural Robustness Affects the Engineerability of Aminoacyl-tRNA Synthetases for Genetic Code Expansion by Grasso, Katherine T, Yeo, Megan J. R, Hillenbrand, Christen M, Ficaretta, Elise D, Italia, James S, Huang, Rachel L, Chatterjee, Abhishek

“…The ability to engineer the substrate specificity of natural aminoacyl-tRNA synthetase/tRNA pairs facilitates the site-specific incorporation of noncanonical…”
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Directed Evolution of a Bacterial Leucyl tRNA in Mammalian Cells for Enhanced Noncanonical Amino Acid Mutagenesis by Huang, Rachel L., Jewel, Delilah, Kelemen, Rachel E., Pham, Quan, Yared, Tarah J., Wang, Shu, Singha Roy, Soumya Jyoti, Huang, Zeyi, Levinson, Samantha D., Sundaresh, Bharathi, Miranda, Suyen Espinoza, van Opijnen, Tim, Chatterjee, Abhishek

“…The Escherichia coli leucyl-tRNA synthetase (EcLeuRS)/tRNAEcLeu pair has been engineered to genetically encode a structurally diverse group of enabling…”
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Enhanced Directed Evolution in Mammalian Cells Yields a Hyperefficient Pyrrolysyl tRNA for Noncanonical Amino Acid Mutagenesis by Jewel, Delilah, Kelemen, Rachel E., Huang, Rachel L., Zhu, Zeyu, Sundaresh, Bharathi, Malley, Kaitlin, Pham, Quan, Loynd, Conor, Huang, Zeyi, Opijnen, Tim, Chatterjee, Abhishek

“…Heterologous tRNAs used for noncanonical amino acid (ncAA) mutagenesis in mammalian cells typically show poor activity. We recently introduced a virus‐assisted…”
Get full text