Search Results - "Huang, Chienjin"

A prospective CSFV-PCV2 bivalent vaccine effectively protects against classical swine fever virus and porcine circovirus type 2 dual challenge and prevents horizontal transmission by Chen, Jing-Yuan, Wu, Chi-Ming, Chia, Min-Yuan, Huang, Chienjin, Chien, Maw-Sheng

“…Classical swine fever virus (CSFV) infection leading to CSF outbreaks is among the most devastating swine diseases in the pig industry. Porcine circovirus type…”
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Efficient expression and purification of porcine circovirus type 2 virus-like particles in Escherichia coli by Wu, Pei-Ching, Chen, Tzu-Yu, Chi, Jiun-Ni, Chien, Maw-Sheng, Huang, Chienjin

“…•Full-length of PCV2 capsid protein is efficiently expressed in E. coli and proceeds to form virus-like particles (VLPs).•The VLPs can be simply purified by a…”
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Detection and phylogenetic analysis of porcine circovirus type 3 in Taiwan by Chang, Chia-Chun, Wu, Ching-Wei, Chang, Yu-Chih, Wu, Ching-Ying, Chien, Maw-Sheng, Huang, Chienjin

“…Porcine circovirus type 3 (PCV3) is a newly emerging porcine circovirus that infects pig populations worldwide. In this study, we investigated the prevalence…”
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Effectiveness of Live-Attenuated Genotype III Japanese Encephalitis Viral Vaccine against Circulating Genotype I Viruses in Swine by Fan, Yi-Chin, Chen, Yi-Ying, Chen, Jo-Mei, Huang, Chienjin, Huang, Mei, Chiou, Shyan-Song

“…Expansion of genotype I (GI) Japanese encephalitis viruses (JEV) has resulted in the replacement of the dominant genotype III (GIII) viruses, raising serious…”
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N-terminus of Classical swine fever virus strain TD96 glycoprotein Erns contains a potential heparin-binding domain by Cheng, Chih-Yuan, Wu, Ching-Wei, Chien, Maw-Sheng, Huang, Chienjin

“…•CSFV Erns was codon optimized and expressed in yeast P. pastoris.•CSFV Erns and C-terminally truncated recombinant proteins bound to heparin.•The N-terminal…”
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The impact of porcine circovirus associated diseases on live attenuated classical swine fever vaccine in field farm applications by Chen, Jing-Yuan, Wu, Chi-Ming, Liao, Chih-Ming, Chen, Kun-Chao, You, Chia-Chin, Wang, Yu-Wen, Huang, Chienjin, Chien, Maw-Sheng

“…Highlights•A vaccination-challenge animal model was conducted to mimic different statuses of PCVADs. •The impaired efficacy of subunit PCV2 vaccine was…”
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Exploring the surface epitope and nuclear localization analysis of porcine circovirus type 3 capsid protein by Chang, Chia-Chun, Wu, Ching-Ying, Ciou, Jhao-Guan, Wu, Ching-Wei, Wang, Yi-Chen, Chang, Hui-Wen, Chien, Maw-Sheng, Huang, Chienjin

“…Porcine circovirus 3 (PCV3) is a newly emerging virus associated with porcine dermatitis and nephropathy syndrome (PDNS) and reproductive disorders, impacting…”
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Characterization of porcine circovirus type 2 (PCV2) capsid particle assembly and its application to virus-like particle vaccine development by Wu, Pei-Ching, Lin, Wei-Li, Wu, Chi-Ming, Chi, Jiun-Ni, Chien, Maw-Sheng, Huang, Chienjin

“…Porcine circovirus type 2 (PCV2) is the primary causative agent of porcine circovirus-associated diseases in pigs. The sole structural capsid protein of PCV2,…”
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Enhancing expression of the classical swine fever virus glycoprotein E2 in yeast and its application to a blocking ELISA by Cheng, Chih-Yuan, Wu, Ching-Wei, Lin, Guang-Jan, Lee, Wei-Cheng, Chien, Maw-Sheng, Huang, Chienjin

“…•Production of yeast-expressed CSFV E2 (yE2) is enhanced by optimizing the first codon.•N-terminal 190 residues of E2 are essential for an efficient…”
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Evaluation of classical swine fever E2 (CSF-E2) subunit vaccine efficacy in the prevention of virus transmission and impact of maternal derived antibody interference in field farm applications by Chen, Jing-Yuan, Wu, Chi-Ming, Chen, Zeng-Weng, Liao, Chih-Ming, Deng, Ming-Chung, Chia, Min-Yuan, Huang, Chienjin, Chien, Maw-Sheng

“…Classical swine fever (CSF) is one of the most devastating pig diseases that affect the swine industry worldwide. Besides stamping out policy for eradication,…”
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Yeast-expressed classical swine fever virus glycoprotein E2 induces a protective immune response by Lin, Guang-Jan, Liu, Ting-Yu, Tseng, Yu-Yao, Chen, Zeng-Weng, You, Chia-Chin, Hsuan, Shih-Ling, Chien, Maw-Sheng, Huang, Chienjin

“…Classical swine fever (CSF) is an economically important swine disease worldwide. The glycoprotein E2 of classical swine fever virus (CSFV) is a viral antigen…”
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Characterization of the monoclonal antibody against classical swine fever virus glycoprotein Erns and its application to an indirect sandwich ELISA by Wu, Ching-Wei, Chien, Maw-Sheng, Liu, Ting-Yu, Lin, Guang-Jan, Lee, Wei-Cheng, Huang, Chienjin

“…Classical swine fever virus (CSFV) E rns is an envelope glycoprotein possessing RNase activity. The E rns -based enzyme-linked immunosorbent assay (ELISA) has…”
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Mechanisms underlying Actinobacillus pleuropneumoniae exotoxin ApxI induced expression of IL-1β, IL-8 and TNF-α in porcine alveolar macrophages by Chen, Zeng-Weng, Chien, Maw-Sheng, Chang, Nai-Yun, Chen, Ter-Hsin, Wu, Chi-Ming, Huang, Chienjin, Lee, Wei-Cheng, Hsuan, Shih-Ling

“…Actinobacillus pleuropneumoniae (A. pleuropneumoniae) causes fibrino-hemorrhagic necrotizing pleuropneumonia in pigs. Production of proinflammatory mediators…”
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Expression of the porcine circovirus type 2 capsid protein subunits and application to an indirect ELISA by Wu, Pei-Ching, Chien, Maw-Sheng, Tseng, Yu-Yao, Lin, Jiarong, Lin, Wei-Li, Yang, Cheng-Yao, Huang, Chienjin

“…Porcine circovirus type 2 (PCV2) is considered to be associated with post-weaning multisystemic wasting syndrome (PMWS), which is a newly emerged economically…”
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Expression and Immunological Studies of Classical Swine Fever Virus Glycoprotein E2 in the Bi-Cistronic Baculovirus/Larvae Expression System by WU, Chi-Ming, HSUAN, Shih-Ling, CHEN, Zeng-Weng, JINN, Tzyy-Rong, HUANG, Chienjin, LIAO, Jiunn-Wang, CHEN, Ter-Hsin, LIAO, Chih-Ming, LEE, Wei-Cheng, WU, Tzong-Yuan, CHIEN, Maw-Sheng

“…To develop an economical, easy technique for producing recombinant E2 glycoprotein (rE2) of classical swine fever virus (CSFV) as a candidate immunogen, a…”
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The N-terminus of porcine circovirus type 2 replication protein is required for nuclear localization and ori binding activities by Lin, Wei-Li, Chien, Maw-Sheng, Du, Yu-Wen, Wu, Pei-Ching, Huang, Chienjin

“…Porcine circovirus type 2 possesses a circular, single-stranded DNA genome that requires the replication protein (Rep) for virus replication. To characterize…”
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Immunogenicity and efficacy of three recombinant subunit Pasteurella multocida toxin vaccines against progressive atrophic rhinitis in pigs by Liao, Chih-Ming, Huang, Chienjin, Hsuan, Shih-Ling, Chen, Zeng-Weng, Lee, Wei-Cheng, Liu, Cheng-I, Winton, James R., Chien, Maw-Sheng

“…Three short fragments of recombinant subunit Pasteurella multocida toxin (rsPMT) were constructed for evaluation as candidate vaccines against progressive…”
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Characterization and expression of the pseudorabies virus early gene UL54 by Huang, Chienjin, Wu, Ching-Ying

“…Pseudorabies virus (PRV) is an alphaherpesvirus, and its gene organization and regulation are similar to the well-characterized human simplex virus (HSV)…”
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Secreted expression of the classical swine fever virus glycoprotein Erns in yeast and application to a sandwich blocking ELISA by CHIENJIN HUANG, CHIEN, Maw-Sheng, HU, Ching-Mei, CHEN, Chung-Wei, HSIEH, Pei-Chen

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Evaluation and application of conducting polymer entrapment on quartz crystal microbalance in flow injection immunoassay by Liu, Yung-Chuan, Wang, Chih-Ming, Hsiung, Kuang-Pin, Huang, Chienjin

“…An immunosensor employing conducting polymer entrapment (CPE) method to immobilize immuno-protein on the quartz crystal microbalance (QCM) for clinical…”
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