Genome-Wide Characterization of Glutathione Peroxidase (GPX) Gene Family in Rapeseed (Brassica napus L.) Revealed Their Role in Multiple Abiotic Stress Response and Hormone Signaling

Plant glutathione peroxidases (GPXs) are the main enzymes in the antioxidant defense system that sustain H2O2 homeostasis and normalize plant reaction to abiotic stress conditions. To understand the major roles of the GPX gene family in rapeseed (Brassica napus L.), for the first time, a genome-wide...

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Bibliographic Details
Published in:	Antioxidants Vol. 10; no. 9; p. 1481
Main Authors:	Li, Wei, Huai, Xuemin, Li, Peitao, Raza, Ali, Mubarik, Muhammad Salman, Habib, Madiha, Fiaz, Sajid, Zhang, Binbin, Pan, Jun, Khan, Rao Sohail Ahmad
Format:	Journal Article
Language:	English
Published:	Basel MDPI AG 17-09-2021 MDPI
Subjects:	Abiotic stress Abscisic acid antioxidant Antioxidants Brassica napus Drought Enzymes gene expression gene ontology Genes Genomes Genomics Glutathione peroxidase Homeostasis Hormones Hydrogen peroxide Oxidative stress Phylogenetics Phylogeny Positive selection Proteins Rape plants Salinity Seeds Software Sorghum Waterlogging Websites Beijing China China
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Summary:	Plant glutathione peroxidases (GPXs) are the main enzymes in the antioxidant defense system that sustain H2O2 homeostasis and normalize plant reaction to abiotic stress conditions. To understand the major roles of the GPX gene family in rapeseed (Brassica napus L.), for the first time, a genome-wide study identified 25 BnGPX genes in the rapeseed genome. The phylogenetic analysis discovered that GPX genes were grouped into four major groups (Group I–Group IV) from rapeseed and three closely interrelated plant species. The universal investigation uncovered that the BnGPXs gene experienced segmental duplications and positive selection pressure. Gene structure and motifs examination recommended that most of the BnGPX genes demonstrated a comparatively well-maintained exon-intron and motifs arrangement within the identical group. Likewise, we recognized five hormones-, four stress-, and numerous light-reactive cis-elements in the promoters of BnGPXs. Five putative bna-miRNAs from two families were also prophesied, targeting six BnGPXs genes. Gene ontology annotation results proved the main role of BnGPXs in antioxidant defense systems, ROS, and response to stress stimulus. Several BnGPXs genes revealed boosted expression profiles in many developmental tissues/organs, i.e., root, seed, leaf, stem, flower, and silique. The qRT-PCR based expression profiling exhibited that two genes (BnGPX21 and BnGPX23) were suggestively up-regulated against different hormones (ABA, IAA, and MeJA) and abiotic stress (salinity, cold, waterlogging, and drought) treatments. In short, our discoveries provide a basis for additional functional studies on the BnGPX genes in future rapeseed breeding programs.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 These authors equally contributed to this work.
ISSN:	2076-3921 2076-3921
DOI:	10.3390/antiox10091481