Picogram determination of an avermectin analog in dog plasma by high-performance liquid chromatography with fluorescence detection. [Erratum: 1997, v. 20 (6), p. 971.]

Picogram determination of an avermectin analog in dog plasma by high-performance liquid chromatography with fluorescence detection. [Erratum: 1997, v. 20 (6), p. 971.]

A sensitive and automated method for the determination of a new avermectin analog (MK-324) in dog plasma has been developed and validated. The drug and internal standard were extracted from plasma via solid-phase extraction using an automated Gilson ASPEC XL system. The extracts were evaporated to d...

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Bibliographic Details
Published in:Journal of liquid chromatography & related technologies Vol. 20; no. 3; pp. 443 - 458
Main Authors: Lin, C.C, Hsieh, J.Y.K, Matuszewski, B.K, Zagrobelny, J, Dobrinska, M.R
Format: Journal Article Conference Proceeding
Language:English
Published: Colchester Taylor & Francis Group 01-01-1997
Taylor & Francis
Subjects:
Analysis
avermectins
Biological and medical sciences
chemical analysis
General pharmacology
high performance liquid chromatography
Medical sciences
Pharmacology. Drug treatments
Pentacyclic compound
Solid phase extraction
Biological fluid
Chemical analysis
HPLC chromatography
Oxygen heterocycle
Blood
Macrocycle
Blood plasma
Percutaneous route
Sample preparation
Parasiticid
Dog
Quantitative analysis
Trace analysis
Fissipedia
Carnivora
Automation
Fluorescence detector
Pesticides
Oral administration
Lactone
Spiran
Vertebrata
Mammalia
Animal
Pharmacokinetics
Topical administration
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Summary:A sensitive and automated method for the determination of a new avermectin analog (MK-324) in dog plasma has been developed and validated. The drug and internal standard were extracted from plasma via solid-phase extraction using an automated Gilson ASPEC XL system. The extracts were evaporated to dryness and reconstituted in a mixture of N,N-diethylmethylamine and acetonitrile. Automated pre-column derivatization of the reconstituted extracts using trifluoroacetic anhydride was performed prior to injection onto the HPLC system. The fluorescent derivatives of the drug and internal standard were chromatographed using a Zorbax RX-C18 column and a mobile phase composed of acetonitrile, water and tetrahydrofuran [6:1:1 (v/v/v)]. The assay was validated in the concentration range of 100 - 5,000 pg/mL. Its applicability, inter-day performance and specificity were demonstrated by analyzing plasma samples from six studies with MK-324.
ISSN:1082-6076
1520-572X
DOI:10.1080/10826079708010662