Evidence against a role for metabotropic glutamate receptors in mossy fiber LTP: the use of mutant mice and pharmacological antagonists

We have used a number of approaches to address a possible role of metabotropic glutamate receptors (mGluRs) in mossy fiber long-term potentiation (LTP) in the hippocampus. We have used two types of mutant mice—one lacking the mGluR1 subtype of receptor and one lacking the gamma isoform of protein ki...

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Published in:	Neuropharmacology Vol. 34; no. 11; pp. 1567 - 1572
Main Authors:	Hsia, A.Y., Salin, P.A., Castillo, P.E., Aiba, A., Abeliovich, A., Tonegawa, S., Nicoll, R.A.
Format:	Journal Article
Language:	English
Published:	England Elsevier Ltd 01-11-1995
Subjects:	Animals Benzoates - pharmacology Excitatory Amino Acid Antagonists - pharmacology Glycine - analogs & derivatives Glycine - pharmacology hippocampus Hippocampus - physiology In Vitro Techniques Long-term potentiation (LTP) Long-Term Potentiation - physiology metabotropic glutamate receptors (mGluRs) Mice Mice, Knockout mossy fibers protein kinase C Protein Kinase C - physiology Receptors, Metabotropic Glutamate - antagonists & inhibitors Receptors, Metabotropic Glutamate - physiology mossy fibers metabotropic glutamate receptors (mGluRs) hippocampus Long-term potentiation (LTP) protein kinase C
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Summary:	We have used a number of approaches to address a possible role of metabotropic glutamate receptors (mGluRs) in mossy fiber long-term potentiation (LTP) in the hippocampus. We have used two types of mutant mice—one lacking the mGluR1 subtype of receptor and one lacking the gamma isoform of protein kinase C. In neither type of mouse did we find any alteration in the magnitude of mossy fiber LTP. We next examined whether mGluRs might modulate the magnitude and/or threshold for the induction of mossy fiber LTP. In these experiments we used tetani that were either just subthreshold or just suprathreshold for generating LTP. The mGluR antagonist (+)-α-methyl-4-carboxyphenylglycine [(+)MCPG] did not convert a subthreshold tetanus into a suprathreshold tetanus, nor did (+)MCPG have any effect on the small amount of LTP that was generated by a just suprathreshold tetanus. Based on our studies, we have been unable to identify a role for mGluRs in mossy fiber LTP.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0028-3908 1873-7064
DOI:	10.1016/0028-3908(95)00115-M