Quantitative Image Analysis of Traumatic Brain Injury Induced Aspiration Pneumonia Treatment
Abstract only Infection of the patient is one of the greatest risks inherent to surgical transplants. While great effort is taken to minimize this risk; variables outside of surgeon control can still result in transference of disease from donor to patient. Such conditions are common in lung transpla...
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| Published in: | The FASEB journal Vol. 31; no. S1 |
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| Main Authors: | , |
| Format: | Journal Article |
| Language: | English |
| Published: |
01-04-2017
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| Online Access: | Get full text |
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| Summary: | Abstract only Infection of the patient is one of the greatest risks inherent to surgical transplants. While great effort is taken to minimize this risk; variables outside of surgeon control can still result in transference of disease from donor to patient. Such conditions are common in lung transplants from donors having suffered traumatic brain injury (TBI). Autonomic nervous system damage from injury prior to transplant can result in involuntary intake of food into the lungs, developing into aspiration pneumonia. Remaining undetected until complications arise in the recipient. This study evaluated TBI model mice treated with simulated transplant conditions. The control groups included: aspiration pneumonia, aspiration treated with phosphate buffered saline (PBS), TBI without aspiration, TBI with aspiration and no treatment, TBI with PBS, TBI with aspiration treated with PBS. Drug treatment groups of TBI with aspiration pneumonia were dosed with either BB5, or a proprietary complement inhibitor. Mouse lung tissue was removed and fixed in 10% neutral buffered formalin (10% NBF) for 24 hours, processed into a paraffin block, cut via microtome and H&E stained. Slides were then examined and whole slide digital images were created and evaluated using quantitative imaging software. The software utilized for calculating the percentage of inflammatory cellular infiltrates across the groups used Red‐Green‐Blue color algorithms to classify stained tissue slides. Initial analysis with Aperio Imagescope was unable to classify the color difference between similarly stained inflammatory cells and alveoli septa in the lungs. Indica Lab's HALO software was used for the second round of analysis. Both drug treatment groups were determined to have a biologically significant reduction in aspiration pneumonia compared to the control groups in both analysis systems. Indica Labs' HALO software was more specific in differentiating inflammatory cells in this difficult analysis. |
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| ISSN: | 0892-6638 1530-6860 |
| DOI: | 10.1096/fasebj.31.1_supplement.983.1 |