Search Results - "Howden, Sara"

Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis by Hou, Zhonggang, Zhang, Yan, Propson, Nicholas E., Howden, Sara E., Chu, Li-Fang, Sontheimer, Erik J., Thomson, James A.

“…Genome engineering in human pluripotent stem cells (hPSCs) holds great promise for biomedical research and regenerative medicine. Recently, an RNA-guided,…”
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Introducing a Spectrum of Long-Range Genomic Deletions in Human Embryonic Stem Cells Using Type I CRISPR-Cas by Dolan, Adam E., Hou, Zhonggang, Xiao, Yibei, Gramelspacher, Max J., Heo, Jaewon, Howden, Sara E., Freddolino, Peter L., Ke, Ailong, Zhang, Yan

“…CRISPR-Cas systems enable microbial adaptive immunity and provide eukaryotic genome editing tools. These tools employ a single effector enzyme of type II or V…”
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Cellular extrusion bioprinting improves kidney organoid reproducibility and conformation by Lawlor, Kynan T., Vanslambrouck, Jessica M., Higgins, J. William, Chambon, Alison, Bishard, Kristina, Arndt, Derek, Er, Pei Xuan, Wilson, Sean B., Howden, Sara E., Tan, Ker Sin, Li, Fanyi, Hale, Lorna J., Shepherd, Benjamin, Pentoney, Stephen, Presnell, Sharon C., Chen, Alice E., Little, Melissa H.

“…Directed differentiation of human pluripotent stem cells to kidney organoids brings the prospect of drug screening, disease modelling and the generation of…”
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3D organoid-derived human glomeruli for personalised podocyte disease modelling and drug screening by Hale, Lorna J., Howden, Sara E., Phipson, Belinda, Lonsdale, Andrew, Er, Pei X., Ghobrial, Irene, Hosawi, Salman, Wilson, Sean, Lawlor, Kynan T., Khan, Shahnaz, Oshlack, Alicia, Quinlan, Catherine, Lennon, Rachel, Little, Melissa H.

“…The podocytes within the glomeruli of the kidney maintain the filtration barrier by forming interdigitating foot processes with intervening slit diaphragms,…”
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Evaluation of variability in human kidney organoids by Phipson, Belinda, Er, Pei X., Combes, Alexander N., Forbes, Thomas A., Howden, Sara E., Zappia, Luke, Yen, Hsan-Jan, Lawlor, Kynan T., Hale, Lorna J., Sun, Jane, Wolvetang, Ernst, Takasato, Minoru, Oshlack, Alicia, Little, Melissa H.

“…The utility of human pluripotent stem cell–derived kidney organoids relies implicitly on the robustness and transferability of the protocol. Here we analyze…”
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Reproducibility and staging of 3D human retinal organoids across multiple pluripotent stem cell lines by Capowski, Elizabeth E, Samimi, Kayvan, Mayerl, Steven J, Phillips, M Joseph, Pinilla, Isabel, Howden, Sara E, Saha, Jishnu, Jansen, Alex D, Edwards, Kimberly L, Jager, Lindsey D, Barlow, Katherine, Valiauga, Rasa, Erlichman, Zachary, Hagstrom, Anna, Sinha, Divya, Sluch, Valentin M, Chamling, Xitiz, Zack, Donald J, Skala, Melissa C, Gamm, David M

“…Numerous protocols have been described for producing neural retina from human pluripotent stem cells (hPSCs), many of which are based on the culture of 3D…”
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Patient-iPSC-Derived Kidney Organoids Show Functional Validation of a Ciliopathic Renal Phenotype and Reveal Underlying Pathogenetic Mechanisms by Forbes, Thomas A., Howden, Sara E., Lawlor, Kynan, Phipson, Belinda, Maksimovic, Jovana, Hale, Lorna, Wilson, Sean, Quinlan, Catherine, Ho, Gladys, Holman, Katherine, Bennetts, Bruce, Crawford, Joanna, Trnka, Peter, Oshlack, Alicia, Patel, Chirag, Mallett, Andrew, Simons, Cas, Little, Melissa H.

“…Despite the increasing diagnostic rate of genomic sequencing, the genetic basis of more than 50% of heritable kidney disease remains unresolved. Kidney…”
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Plasticity of distal nephron epithelia from human kidney organoids enables the induction of ureteric tip and stalk by Howden, Sara E., Wilson, Sean B., Groenewegen, Ella, Starks, Lakshi, Forbes, Thomas A., Tan, Ker Sin, Vanslambrouck, Jessica M., Holloway, Emily M., Chen, Yi-Hsien, Jain, Sanjay, Spence, Jason R., Little, Melissa H.

“…During development, distinct progenitors contribute to the nephrons versus the ureteric epithelium of the kidney. Indeed, previous human pluripotent…”
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Enhanced metanephric specification to functional proximal tubule enables toxicity screening and infectious disease modelling in kidney organoids by Vanslambrouck, Jessica M., Wilson, Sean B., Tan, Ker Sin, Groenewegen, Ella, Rudraraju, Rajeev, Neil, Jessica, Lawlor, Kynan T., Mah, Sophia, Scurr, Michelle, Howden, Sara E., Subbarao, Kanta, Little, Melissa H.

“…While pluripotent stem cell-derived kidney organoids are now being used to model renal disease, the proximal nephron remains immature with limited evidence for…”
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Renal Subcapsular Transplantation of PSC-Derived Kidney Organoids Induces Neo-vasculogenesis and Significant Glomerular and Tubular Maturation In Vivo by van den Berg, Cathelijne W., Ritsma, Laila, Avramut, M. Cristina, Wiersma, Loes E., van den Berg, Bernard M., Leuning, Daniëlle G., Lievers, Ellen, Koning, Marije, Vanslambrouck, Jessica M., Koster, Abraham J., Howden, Sara E., Takasato, Minoru, Little, Melissa H., Rabelink, Ton J.

“…Human pluripotent stem cell (hPSC)-derived kidney organoids may facilitate disease modeling and the generation of tissue for renal replacement. Long-term…”
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Forward steps in organoid-based forward screening by Little, Melissa H., Howden, Sara E.

“…In this issue of Cell Stem Cell, Ungricht et al. (2022) perform a temporally controlled CRISPR/Cas9-based genome-wide screen in kidney organoids to uncover key…”
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A Cas9 Variant for Efficient Generation of Indel-Free Knockin or Gene-Corrected Human Pluripotent Stem Cells by Howden, Sara E., McColl, Bradley, Glaser, Astrid, Vadolas, Jim, Petrou, Steven, Little, Melissa H., Elefanty, Andrew G., Stanley, Edouard G.

“…While Cas9 nucleases permit rapid and efficient generation of gene-edited cell lines, the CRISPR-Cas9 system can introduce undesirable “on-target” mutations…”
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Chemically defined conditions for human iPSC derivation and culture by Chen, Guokai, Gulbranson, Daniel R, Hou, Zhonggang, Bolin, Jennifer M, Ruotti, Victor, Probasco, Mitchell D, Smuga-Otto, Kimberly, Howden, Sara E, Diol, Nicole R, Propson, Nicholas E, Wagner, Ryan, Lee, Garrett O, Antosiewicz-Bourget, Jessica, Teng, Joyce M C, Thomson, James A

“…A defined and simplified culture system for the derivation and growth of human induced pluripotent stem cells is reported. It permits increased efficiency of…”
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Optic Vesicle‐like Structures Derived from Human Pluripotent Stem Cells Facilitate a Customized Approach to Retinal Disease Treatment by Meyer, Jason S., Howden, Sara E., Wallace, Kyle A., Verhoeven, Amelia D., Wright, Lynda S., Capowski, Elizabeth E., Pinilla, Isabel, Martin, Jessica M., Tian, Shulan, Stewart, Ron, Pattnaik, Bikash, Thomson, James A., Gamm, David M.

“…Differentiation methods for human induced pluripotent stem cells (hiPSCs) typically yield progeny from multiple tissue lineages, limiting their use for drug…”
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Kidney Organoids Generated Using an Allelic Series of NPHS2 Point Variants Reveal Distinct Intracellular Podocin Mistrafficking by Dorison, Aude, Ghobrial, Irene, Graham, Alison, Peiris, Thanushi, Forbes, Thomas A, See, Michael, Das, Mithun, Saleem, Moin A, Quinlan, Catherine, Lawlor, Kynan T, Ramialison, Mirana, Howden, Sara E, Little, Melissa H

“…NPHS2 variants are the most common cause of steroid-resistant nephrotic syndrome in children >1 month old. Missense NPHS2 variants were reported to cause…”
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Reporter‐based fate mapping in human kidney organoids confirms nephron lineage relationships and reveals synchronous nephron formation by Howden, Sara E, Vanslambrouck, Jessica M, Wilson, Sean B, Tan, Ker Sin, Little, Melissa H

“…Nephron formation continues throughout kidney morphogenesis in both mice and humans. Lineage tracing studies in mice identified a self‐renewing Six2‐expressing…”
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Simultaneous Reprogramming and Gene Correction of Patient Fibroblasts by Howden, Sara E., Maufort, John P., Duffin, Bret M., Elefanty, Andrew G., Stanley, Edouard G., Thomson, James A.

“…The derivation of genetically modified induced pluripotent stem (iPS) cells typically involves multiple steps, requiring lengthy cell culture periods, drug…”
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Functional Assessment of Patient-Derived Retinal Pigment Epithelial Cells Edited by CRISPR/Cas9 by Foltz, Leah P, Howden, Sara E, Thomson, James A, Clegg, Dennis O

“…Retinitis pigmentosa is the most common form of inherited blindness and can be caused by a multitude of different genetic mutations that lead to similar…”
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Generating Kidney from Stem Cells by Little, Melissa H, Hale, Lorna J, Howden, Sara E, Kumar, Santhosh V

“…Human kidney tissue can now be generated via the directed differentiation of human pluripotent stem cells. This advance is anticipated to facilitate the…”
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Generating Kidney Organoids from Human Pluripotent Stem Cells Using Defined Conditions by Howden, Sara E, Little, Melissa H

“…The ultimate goal of regenerative medicine is to have access to an unlimited supply of specific cell types on demand, which can be used as effective therapies…”
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