Search Results - "Housden, Benjamin E."

Comparison of Cas9 activators in multiple species by Chavez, Alejandro, Tuttle, Marcelle, Pruitt, Benjamin W, Ewen-Campen, Ben, Chari, Raj, Ter-Ovanesyan, Dmitry, Haque, Sabina J, Cecchi, Ryan J, Kowal, Emma J K, Buchthal, Joanna, Housden, Benjamin E, Perrimon, Norbert, Collins, James J, Church, George

“…A comparison of seven dCas9-based transcriptional activators shows that VPR, SAM, and Suntag perform best in cell lines from a variety of organisms. Several…”
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In Vivo Transcriptional Activation Using CRISPR/Cas9 in Drosophila by Lin, Shuailiang, Ewen-Campen, Ben, Ni, Xiaochun, Housden, Benjamin E, Perrimon, Norbert

“…A number of approaches for Cas9-mediated transcriptional activation have recently been developed, allowing target genes to be overexpressed from their…”
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Highly efficient Cas9-mediated transcriptional programming by Chavez, Alejandro, Scheiman, Jonathan, Vora, Suhani, Pruitt, Benjamin W, Tuttle, Marcelle, P R Iyer, Eswar, Lin, Shuailiang, Kiani, Samira, Guzman, Christopher D, Wiegand, Daniel J, Ter-Ovanesyan, Dmitry, Braff, Jonathan L, Davidsohn, Noah, Housden, Benjamin E, Perrimon, Norbert, Weiss, Ron, Aach, John, Collins, James J, Church, George M

“…The fusion of three transcriptional activation domains to a nuclease-deficient Cas9 achieves robust induction of gene expression and can induce differentiation…”
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Spatial and temporal organization of signaling pathways by Housden, Benjamin E., Perrimon, Norbert

“…•Signaling pathways produce diverse outcomes following apparently similar stimulation.•The context, crosstalk, and dynamics of signaling are important in…”
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Loss-of-function genetic tools for animal models: cross-species and cross-platform differences by Housden, Benjamin E., Muhar, Matthias, Gemberling, Matthew, Gersbach, Charles A., Stainier, Didier Y. R., Seydoux, Geraldine, Mohr, Stephanie E., Zuber, Johannes, Perrimon, Norbert

“…Key Points Loss-of-function (LOF) technologies are widely used across many model organisms and many fields. Although all LOF approaches have the shared goal of…”
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Optimized gene editing technology for Drosophila melanogaster using germ line-specific Cas9 by Ren, Xingjie, Sun, Jin, Housden, Benjamin E., Hu, Yanhui, Roesel, Charles, Lin, Shuailiang, Liu, Lu-Ping, Yang, Zhihao, Mao, Decai, Sun, Lingzhu, Wu, Qujie, Jie, Jun-Yuan, Xi, Jianzhong, Mohr, Stephanie E., Xu, Jiang, Perrimon, Norbert, Ni, Jian-Quan

“…The ability to engineer genomes in a specific, systematic, and cost-effective way is critical for functional genomic studies. Recent advances using the…”
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CRISPR guide RNA design for research applications by Mohr, Stephanie E., Hu, Yanhui, Ewen‐Campen, Benjamin, Housden, Benjamin E., Viswanatha, Raghuvir, Perrimon, Norbert

“…The rapid rise of CRISPR as a technology for genome engineering and related research applications has created a need for algorithms and associated online tools…”
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A gene-specific T2A-GAL4 library for Drosophila by Lee, Pei-Tseng, Zirin, Jonathan, Kanca, Oguz, Lin, Wen-Wen, Schulze, Karen L, Li-Kroeger, David, Tao, Rong, Devereaux, Colby, Hu, Yanhui, Chung, Verena, Fang, Ying, He, Yuchun, Pan, Hongling, Ge, Ming, Zuo, Zhongyuan, Housden, Benjamin E, Mohr, Stephanie E, Yamamoto, Shinya, Levis, Robert W, Spradling, Allan C, Perrimon, Norbert, Bellen, Hugo J

“…We generated a library of ~1000 stocks in which we inserted a construct in the intron of genes allowing expression of under control of endogenous promoters…”
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Seipin is required for converting nascent to mature lipid droplets by Wang, Huajin, Becuwe, Michel, Housden, Benjamin E, Chitraju, Chandramohan, Porras, Ashley J, Graham, Morven M, Liu, Xinran N, Thiam, Abdou Rachid, Savage, David B, Agarwal, Anil K, Garg, Abhimanyu, Olarte, Maria-Jesus, Lin, Qingqing, Fröhlich, Florian, Hannibal-Bach, Hans Kristian, Upadhyayula, Srigokul, Perrimon, Norbert, Kirchhausen, Tomas, Ejsing, Christer S, Walther, Tobias C, Farese, Robert V

“…How proteins control the biogenesis of cellular lipid droplets (LDs) is poorly understood. Using and human cells, we show here that seipin, an ER protein…”
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Exploring the Role of Reactive Oxygen Species in the Pathogenesis and Pathophysiology of Alzheimer's and Parkinson's Disease and the Efficacy of Antioxidant Treatment by Gogna, Talin, Housden, Benjamin E, Houldsworth, Annwyne

“…Alzheimer's (AD) and Parkinson's Disease (PD) are life-altering diseases that are characterised by progressive memory loss and motor dysfunction. The…”
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The Septate Junction Protein Tsp2A Restricts Intestinal Stem Cell Activity via Endocytic Regulation of aPKC and Hippo Signaling by Xu, Chiwei, Tang, Hong-Wen, Hung, Ruei-Jiun, Hu, Yanhui, Ni, Xiaochun, Housden, Benjamin E., Perrimon, Norbert

“…Hippo signaling and the activity of its transcriptional coactivator, Yorkie (Yki), are conserved and crucial regulators of tissue homeostasis. In the…”
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Context-Dependent Enhancer Selection Confers Alternate Modes of Notch Regulation on argos by Housden, Benjamin E., Terriente-Felix, Ana, Bray, Sarah J.

“…OA  Classifications Services MCB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley…”
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Zinc Detoxification: A Functional Genomics and Transcriptomics Analysis in Drosophila melanogaster Cultured Cells by Mohr, Stephanie E, Rudd, Kirstin, Hu, Yanhui, Song, Wei Roc, Gilly, Quentin, Buckner, Michael, Housden, Benjamin E, Kelley, Colleen, Zirin, Jonathan, Tao, Rong, Amador, Gabriel, Sierzputowska, Katarzyna, Comjean, Aram, Perrimon, Norbert

“…Cells require some metals, such as zinc and manganese, but excess levels of these metals can be toxic. As a result, cells have evolved complex mechanisms for…”
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Comparing CRISPR and RNAi-based screening technologies by Housden, Benjamin E, Perrimon, Norbert

“…Two studies provide an experimental side-by-side comparison of genetic screening methods…”
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A Mechanism Coupling Systemic Energy Sensing to Adipokine Secretion by Rajan, Akhila, Housden, Benjamin E., Wirtz-Peitz, Frederik, Holderbaum, Laura, Perrimon, Norbert

“…Adipocytes sense systemic nutrient status and systemically communicate this information by releasing adipokines. The mechanisms that couple nutritional state…”
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Mapping signaling pathway cross-talk in Drosophila cells by Ammeux, Noemie, Housden, Benjamin E., Georgiadis, Andrew, Hu, Yanhui, Perrimon, Norbert

“…During development and homeostasis, cells integrate multiple signals originating either from neighboring cells or systemically. In turn, responding cells can…”
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mTORC1 Couples Nucleotide Synthesis to Nucleotide Demand Resulting in a Targetable Metabolic Vulnerability by Valvezan, Alexander J., Turner, Marc, Belaid, Amine, Lam, Hilaire C., Miller, Spencer K., McNamara, Molly C., Baglini, Christian, Housden, Benjamin E., Perrimon, Norbert, Kwiatkowski, David J., Asara, John M., Henske, Elizabeth P., Manning, Brendan D.

“…The mechanistic target of rapamycin complex 1 (mTORC1) supports proliferation through parallel induction of key anabolic processes, including protein, lipid,…”
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Resources for functional genomics studies in Drosophila melanogaster by Mohr, Stephanie E, Hu, Yanhui, Kim, Kevin, Housden, Benjamin E, Perrimon, Norbert

“…Drosophila melanogaster has become a system of choice for functional genomic studies. Many resources, including online databases and software tools, are now…”
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Cas9-based genome editing in Drosophila by Housden, Benjamin E, Lin, Shuailiang, Perrimon, Norbert

“…Our ability to modify the Drosophila genome has recently been revolutionized by the development of the CRISPR system. The simplicity and high efficiency of…”
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Emergent RNA–RNA interactions can promote stability in a facultative phototrophic endosymbiosis by Jenkins, Benjamin H., Maguirec, Finlay, Leonard, Guy, Eaton, Joshua D., West, Steven, Housden, Benjamin E., Milner, David S., Richards, Thomas A.

“…Eukaryote–eukaryote endosymbiosiswas responsible for the spread of chloroplast (plastid) organelles. Stability is required for the metabolic and genetic…”
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