Decreased miR-320a promotes invasion and metastasis of tumor budding cells in tongue squamous cell carcinoma

We aimed to determine the specific miRNA profile of tumor budding cells and investigate the potential role of miR-320a in invasion and metastasis of tongue squamous cell carcinoma (TSCC). We collected tumor budding cells and paired central tumor samples from five TSCC specimens with laser capture mi...

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Bibliographic Details
Published in:	Oncotarget Vol. 7; no. 40; pp. 65744 - 65757
Main Authors:	Xie, Nan, Wang, Cheng, Zhuang, Zehang, Hou, Jinson, Liu, Xiqiang, Wu, Yue, Liu, Haichao, Huang, Hongzhang
Format:	Journal Article
Language:	English
Published:	United States Impact Journals LLC 04-10-2016
Subjects:	Animals Apoptosis Biomarkers, Tumor - genetics Carcinoma, Squamous Cell - genetics Carcinoma, Squamous Cell - metabolism Carcinoma, Squamous Cell - secondary Cell Proliferation Female Follow-Up Studies Gene Expression Regulation, Neoplastic Humans Lymphatic Metastasis Male Mice Mice, Inbred BALB C Mice, Nude MicroRNAs - genetics Middle Aged Neoplasm Invasiveness Polycomb Repressive Complex 2 - metabolism Prognosis Research Paper Survival Rate Tongue Neoplasms - genetics Tongue Neoplasms - metabolism Tongue Neoplasms - pathology Tumor Cells, Cultured Xenograft Model Antitumor Assays Suz12 miR-320a invasion and metastasis tumor budding tongue squamous cell carcinoma
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Summary:	We aimed to determine the specific miRNA profile of tumor budding cells and investigate the potential role of miR-320a in invasion and metastasis of tongue squamous cell carcinoma (TSCC). We collected tumor budding cells and paired central tumor samples from five TSCC specimens with laser capture microdissection and examined the specimens using a miRNA microarray. The specific miRNA signature of tumor budding cells was identified. We found that miR-320a was dramatically decreased in tumor budding cells. Knockdown of miR-320a significantly enhanced migration and invasion of TSCC cell lines. Suz12 was shown to be a direct target of miR-320a. Similar results were also observed in nude mouse models. Multivariate analysis indicated that miR-320a was an independent prognostic factor. Kaplan-Meier analysis demonstrated that decreased miR-320a and high intensity of tumor budding were correlated with poor survival rate, especially in the subgroup with high-intensity tumor budding and low expression of miR-320a. We concluded that decreased expression of miR-320a could promote invasion and metastasis of tumor budding cells by targeting Suz12 in TSCC. A combination of tumor budding and miR-320a may serve as an index to identify an aggressive sub-population of TSCC cells with high metastatic potential.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1949-2553 1949-2553
DOI:	10.18632/oncotarget.11612