Ionic Liquid Modification Optimizes the Interface between Lipase and Magnetic GO for Enhancing Biocatalysis

In this study, imidazolium-based ionic liquids (ILs) were used to modify carboxymethyl cellulose-coated Fe3O4 nanoparticles with graphene oxide deposition (ILs-MCMC/GO). The prepared composites were characterized and used to immobilize lipase. The prepared biocatalyst (PPL-ILs-MCMC/GO) exhibited hig...

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Bibliographic Details
Published in:	Industrial & engineering chemistry research Vol. 61; no. 3; pp. 1277 - 1284
Main Authors:	Xu, Lili, Liu, Renmin, Li, Zhaofang, Li, Moju, Zhao, Min, Li, Yingjie, Hou, Guiyue, Li, Aifeng, Suo, Hongbo
Format:	Journal Article
Language:	English
Published:	American Chemical Society 26-01-2022
Subjects:	Bioengineering
Online Access:	Get full text
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Summary:	In this study, imidazolium-based ionic liquids (ILs) were used to modify carboxymethyl cellulose-coated Fe3O4 nanoparticles with graphene oxide deposition (ILs-MCMC/GO). The prepared composites were characterized and used to immobilize lipase. The prepared biocatalyst (PPL-ILs-MCMC/GO) exhibited higher activity, which was 3.55-fold that of free lipase and 1.41-fold that of immobilized lipase without IL (PPL-MCMC/GO). After reusing 10 times, the residual activity of PPL-ILs-MCMC/GO was 91.3%, which was higher than those of PPL-MCMC/GO (83.6%) and PPL-MCMC (73.6%). In addition, using kinetic analysis, we showed that the affinity between PPL-ILs-MCMC/GO and the substrate was improved. The secondary structures of free and immobilized lipase were analyzed to explain the mechanism by which the performance of PPL-ILs-MCMC/GO was improved. The introduction of imidazole-based IL was useful in improving the microenvironment of immobilized lipase. Furthermore, magnetic supports loaded with lipase could be recovered easily.
ISSN:	0888-5885 1520-5045
DOI:	10.1021/acs.iecr.1c04396