Search Results - "Hossler, Patrick"
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Adeno-associated virus perfusion enhanced expression: A commercially scalable, high titer, high quality producer cell line process
Published in Molecular therapy. Methods & clinical development (13-06-2024)“…With safety and efficacy demonstrated over hundreds of clinical trials in the last 30 years, along with at least six recent global marketing authorizations…”
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Cell culture media supplementation of bioflavonoids for the targeted reduction of acidic species charge variants on recombinant therapeutic proteins
Published in Biotechnology progress (01-07-2015)“…Charge variants in recombinant proteins are an important series of protein modifications, whose potential role on protein stability, activity, immunogenicity,…”
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Journal Article -
3
Systems analysis of N-glycan processing in mammalian cells
Published in PloS one (08-08-2007)“…N-glycosylation plays a key role in the quality of many therapeutic glycoprotein biologics. The biosynthesis reactions of these oligosaccharides are a type of…”
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4
Arabinosylation of recombinant human immunoglobulin-based protein therapeutics
Published in mAbs (19-05-2017)“…Protein glycosylation is arguably the paramount post-translational modification on recombinant glycoproteins, and highly cited in the literature for affecting…”
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Journal Article -
5
Improvement of mammalian cell culture performance through surfactant enabled concentrated feed media
Published in Biotechnology progress (01-07-2013)“…The design of basal and feed media in mammalian cell culture is paramount towards ensuring acceptable upstream process performance in various operation modes,…”
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Protein glycosylation control in mammalian cell culture: past precedents and contemporary prospects
Published in Advances in biochemical engineering, biotechnology (01-01-2012)“…Protein glycosylation is a post-translational modification of paramount importance for the function, immunogenicity, and efficacy of recombinant glycoprotein…”
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Optimal and consistent protein glycosylation in mammalian cell culture
Published in Glycobiology (Oxford) (01-09-2009)“…In the biopharmaceutical industry, mammalian cell culture systems, especially Chinese hamster ovary (CHO) cells, are predominantly used for the production of…”
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Cell culture media supplementation of infrequently used sugars for the targeted shifting of protein glycosylation profiles
Published in Biotechnology progress (01-03-2017)“…Mammalian cells in culture rely on sources of carbohydrates to supply the energy requirements for proliferation. In addition, carbohydrates provide a large…”
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Sustaining an efficient and effective CHO cell line development platform by incorporation of 24‐deep well plate screening and multivariate analysis
Published in Biotechnology progress (01-01-2018)“…Efficient and effective cell line screening is paramount toward a successful biomanufacturing program. Here we describe the implementation of 24‐deep well…”
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When Good Intentions Go Awry: Modification of a Recombinant Monoclonal Antibody in Chemically Defined Cell Culture by Xylosone, an Oxidative Product of Ascorbic Acid
Published in Analytical chemistry (Washington) (04-08-2015)“…With the advent of new initiatives to develop chemically defined media, cell culture scientists screen many additives to improve cell growth and productivity…”
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Cell culture media supplementation of uncommonly used sugars sucrose and tagatose for the targeted shifting of protein glycosylation profiles of recombinant protein therapeutics
Published in Biotechnology progress (01-11-2014)“…Protein glycosylation is an important post‐translational modification toward the structure and function of recombinant therapeutics. The addition of…”
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Engineering cells for cell culture bioprocessing--physiological fundamentals
Published in Advances in biochemical engineering, biotechnology (01-01-2006)“…In the past decade, we have witnessed a tremendous increase in the number of mammalian cell-derived therapeutic proteins with clinical applications. The…”
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Journal Article -
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GlycoVis: Visualizing glycan distribution in the protein N-glycosylation pathway in mammalian cells
Published in Biotechnology and bioengineering (05-12-2006)“…Glycosylation has profound effects on the quality of recombinant proteins produced in mammalian cells. The biosynthetic pathways of N‐linked glycans on…”
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Journal Article -
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Experimental and theoretical exploration of protein glycosylation in mammalian cell culture
Published 01-01-2006“…Mammalian cells have been successfully used for the production of various recombinant proteins. Mammalian cell metabolism is highly dependent on a multitude of…”
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Dissertation
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15
Arabinosylation of recombinant human immunoglobulin-based protein therapeutics
Published in mAbs (19-05-2017)“…Protein glycosylation is arguably the paramount post-translational modification on recombinant glycoproteins, and highly cited in the literature for affecting…”
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Report